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Table 1 Fundamental information of 119 differentially expressed protein spots identified by MS

From: Analysis of protein expression profiles in the thymus of chickens infected with Marek’s disease virus

SSP Protein Name Fold change in expression Accession number MW PI
   4dpi 7dpi 14dpi 21dpi 28dpi 35dpi 42dpi    
Actin cytoskeleton and cellular structural proteins
3908 collagen alpha-1(VI) chain precursor −1.05 1.57 1.44 4.53** 3.47** 1.14 −1.65 gi|49225581 107916.8 5.63
3910 collagen alpha-1(VI) chain precursor 1.32 2.92** −1.66 3.77** 3.72* −1.73 1.06 gi|49225581 107916.8 5.63
3914 collagen alpha-2(VI) chain precursor 1.01 1.79 1.54 4.11** −1.2 1.35 −1.01 gi|45384382 109108.4 5.66
3915 collagen alpha-2(VI) chain precursor −1.33 2.24** 1.4 3.58** 1.71 1.89 1.09 gi|45384382 109108.4 5.66
508 keratin, type I cytoskeletal 15 −1.22 −1.17 1.06 2.67** 1.28 −1.02 −1.03 gi|47604932 48060.8 5.11
6713 keratin, type II cytoskeletal 1 −1.03 −1.68 1.23 −2.25** −1.43 −1.2 −1.69 gi|119395750 65999 8.15
4502 keratin, type II cytoskeletal cochleal 1.69 −1.15 −2.55** −1.2 1.64 1.42 −1.23 gi|45384378 53770.5 5.97
106 Chain B, Refined 1.8 Angstroms Resolution Crystal Structure Of Porcine Epsilon-Trypsin 1.34 −1.31 −1.14 −2.62** −2.28** −2.63** −1.29 gi|999627 8813.5 6.67
9222 PREDICTED: LOW QUALITY PROTEIN: keratin, type II cytoskeletal 1-like AB IN AB AB AB AB AB gi|296211766 66379 8.16
6206 REDICTED: similar to Chain D, Crystal Structure Of Arp23 COMPLEX 1.08 1.34 1.07 −1.04 −1.08 1.21 2.14** gi|118093746 34379.6 6.84
6211 PREDICTED: similar to Chain D, Crystal Structure Of Arp23 COMPLEX −2.11** 3.72** 1.09 4.76** 2.22** 4.28** 1.73 gi|118093746 34379.6 6.84
314 beta-tropomyosin −1.19 1.04 1.37 1.82 1.66 2.86** 1.43 gi|212809 32779.7 4.77
6601 fibrinogen beta chain precursor −1.37 1.28 −1.01 1.88 2.33** −1.2 −1.44 gi|267844833 54545.7 7.84
2606 plastin-2 −1.65 2.04** 1.23 2.44** 2.24* 2.13** 1.27 gi|56605886 69704.5 5.16
2206 beta-actin −1.1 1.87 1.08 1.06 1.53 2.08** 2.2** gi|154818367 41812.8 5.32
3906 collagen alpha-1(VI) chain precursor −1.07 1.67 1.51 4.29** 1.68 1.55 −1.61 gi|49225581 107916.8 5.63
Enzymes
4406 adenylosuccinate synthetase isozyme 2 −1.29 1.1 −1.39 2.19* 2.2* −1.46 −1.16 gi|71895783 49423.4 5.93
4410 argininosuccinate synthase 1.48 1.56 1.49 IN 3.99 2.64** −1.2 gi|61657937 46873.8 6.1
5402 argininosuccinate synthase −1.63 4.04** 1.03 1.15 1 1 −1.45 gi|61657937 46873.8 6.1
5904 argininosuccinate synthase 1.35 2.49** 1.22 1.82 1.83 −1.23 −1.67 gi|61657937 46873.8 6.1
6705 ATP-dependent RNA helicase DDX3X −1.03 −1.33 1.15 −2.2** −2.27** 1 −1.55 gi|71895253 72004.5 6.54
3414 B-creatine kinase −1.32 1.73 −1.3 2.09** 1.76 1.34 −1.08 gi|211235 42240.4 5.78
7213 L-lactate dehydrogenase A chain −1.08 1.71 −1.03 2.36** 2.63** 2.43** −1.4 gi|45384208 36491.2 7.75
6606 phosphoglucomutase 2 AB REP REP −1.36 AB −1.4 1.29 gi|71897287 67900.1 6.56
9221 PREDICTED: similar to 2,4-dienoyl-CoA reductase AB IN AB AB AB AB AB gi|50731694 35723.5 9.46
3205 PREDICTED: similar to pyridoxal kinase AB 1.56 1.45 2.74* 2.02** 2.45** −1.04 gi|118083963 43075.8 8.36
3207 RecName: Full=3-mercaptopyruvate sulfurtransferase; Short=MST AB AB IN AB AB AB AB gi|90110410 33002.5 6.11
6107 RecName: Full=Carbonic anhydrase 2; AltName: −1.13 −1.45 1.13 1.38 3.04 1.19 2.22* gi|115454 29388.4 6.56
Full=Carbonate dehydratase II; AltName: Full=Carboni anhydrase II; Short=CA-II
2622 PREDICTED: similar to serine/threonine kinase isoform 1 AB 1.17 1.44 REP −1.22 −1.43 −2.46** gi|118094908 57883.8 6.04
4117 PREDICTED: similar to Glyoxylase 1 REP AB AB AB AB AB AB gi|50740506 20540.2 6.1
6402 creatine kinase M-type AB 1.16 1.32 2.64* 2.68** 2.47** −1.04 gi|45382875 43301 6.5
6408 creatine kinase M-type −1.39 2.07* 1.75 6.7** 1.14 2.27 −1.06 gi|45382875 43301 6.5
6414 enolase 1.24 1.18 1.28 1.59 2.38** 1.4 1.25 gi|116248308 41047.2 5.62
9101 glutathione S-transferase 1.04 1.99 1.39 2.47** 2.7** 2.58** −1.13 gi|49169816 25282.4 8.86
4210 PREDICTED: gemin 4 −1.58 1.18 1.56 −1.24 1.07 −2.17** −1.37 gi|224076562 30154.9 4.96
1109 cathepsin B precursor 1.02 −1.03 1.04 2.27** 2.39** 2.86** 1.04 gi|46195455 37562.7 5.74
5607 peptidase D −1.39 1.49 IN 1.73 IN 2.08** −1.21 gi|169139269 55101.6 6.07
9111 peptidyl-prolyl cis-trans isomerase B precursor 1.15 1.55 1.12 2** 1.78 1.88 −1.17 gi|45382027 22398.8 9.4
6111 PREDICTED: similar to Glutathione S-transferase theta 1 isoform 3 1.55 −1.1 −1.59 −2.38** −1.56 −3.36** 1.13 gi|118098704 27843.7 6.25
Immunity, apoptosis, tumor development and viral infection and replication
6507 septin-6 −1.15 −1.27 −6.03** −1.82 −1.35 −1.39 1.2 gi|71895629 48656.9 6.67
6521 septin-6 1.1 1.25 2.42** −1.3 −1.05 −1.15 −1.55 gi|71895629 43021.1 6.63
7310 septin-9 −1.08 −1.38 −1.08 −2.87** −2.09* −2.33* −1.25 gi|71897123 65482.8 8.42
3203 PREDICTED: similar to PTB-associated splicing factor AB AB −2.1** AB −2.75** −1.09 2.06** gi|118101662 69173.9 9.43
7116 PREDICTED: heterogeneous nuclear ribonucleoprotein A3 −1.97 2.16** −1.67 11.96** 2.33** 2.4** 1.4 gi|118093536 40603.2 9.24
8310 PREDICTED: heterogeneous nuclear ribonucleoprotein A3 AB AB AB −6.86** AB AB AB gi|118093536 40603.2 9.24
1310 PREDICTED: heterogeneous nuclearribonucleoproteins C1/C2-like −1.22 −1.19 −1.04 −3.14** −2.43* −1.36 1.29 gi|296224780 23551.2 9.87
8304 heterogeneous nuclear ribonucleoprotein A/B 1.02 1.29 −1.92 −2.39** −2.58** −2.11** −1.18 gi|45384514 31841.8 8.62
2613 heterogeneous nuclear ribonucleoprotein H −1.14 −1.78 1.87 −2.13** −3.36 −2.48 1.05 gi|45383173 56530.6 5.38
2610 heterogeneous nuclear ribonucleoprotein H 1.22 −2.25* −1.31 AB −2.11** −1.82 −2.91 gi|45383173 56530.6 5.38
3101 heterogeneous nuclear ribonucleoproteins A2/B1 1.69 1.46 −1.39 −1.6 −2.00** 1.79 1.07 gi|71896753 36962.4 8.67
8211 heterogeneous nuclear ribonucleoproteins A2/B1 −1.1 −1.11 1.06 −2** −2** −1.68 −1.12 gi|71896753 36962.4 8.67
8213 heterogeneous nuclear ribonucleoproteins A2/B1 1.04 −1.07 −1.46 −2.53** −1.41 −2.01** −1.1 gi|71896753 36962.4 8.67
9205 heterogeneous nuclear ribonucleoproteins A2/B1 −1.08 1.07 −1.41 −2.47** −2.19** −1.57 −1.12 gi|71896753 36962.4 8.67
7006 macrophage migration inhibitory factor 1.35 −1.03 −2.04** −1.64 −1.77 −1.84 1.74 gi|212258 12704.2 6.88
2905 heat shock protein HSP 90-alpha −1.37 −1.55 −1.23 −3.73** −1.58 −1.63 −1.67 gi|157954047 84006.5 5.01
7215 annexin A1 AB IN 1 IN IN IN AB gi|46195459 38475.9 7.05
7703 far upstream element-binding protein 1 1.03 −2.56 1.34 −2.53** −2.38* −1.85 −1.48 gi|83320094 67155.3 7.18
3014 stathmin 1.19 1 −1.16 −4.27** −7.72** −7.18** 1.05 gi|50053682 17071.9 6.18
4003 stathmin 1.1 −2.1** −1.1 −4.91** −4.49** −5.08** −1.1 gi|50053682 17071.9 6.18
405 vimentin −1.5 3.27* 1.56 3.31** 1.36 1.06 1.04 gi|114326309 53109.6 5.09
1605 vimentin 1.62 1.76 1.77 2.9** −1.35 −1.08 −1.01 gi|114326309 53109.6 5.09
409 vimentin 1.23 1.64 1.42 2.26** 1.47 1.31 1 gi|114326309 53109.6 5.09
205 nucleolar protein B23/No38 1.28 1.76 −1.51 1.54 1.58 1.65 6.95** gi|212456 10716.2 4.38
6003 beta-galactoside-binding lectin 1.07 2.22** 1.56 2.7** 2.66** 2.4** −1.45 gi|45382785 15053.5 6.58
3113 PREDICTED: similar to natural killer cell enhancing factor isoform 4 1.63 1.12 1.63* 1.58 2.5** 2.28** −1.27 gi|50751518 22300.5 8.24
5304 unnamed protein product 1.23 1.88 1.45 −1.49 −1.26 1.28 2.11** gi|74142813 50433.1 6.17
1104 PREDICTED: similar to interferon, gamma-inducible protein 30 1.14 −1.05 −1.04 −2.27** −1.29 −1.12 1.19 gi|50761132 14269.9 5.73
401 mCG49244 1.23 −1.56 1.27 −2.25* −2.04** −1.63 −1.73 gi|148694498 21747.1 5.71
114 SET 1.62 5.16** 1.18 −1.01 −1.07 1.01 1.51 gi|3953617 24348 4.97
Mitosis, replication and translation
1402 put. beta-actin (aa 27–375) 1.01 −1.27 −2.74** −13.22** −14.21** −1.1 −1.07 gi|49868 39160.6 5.78
2303 suppressor of G2 allele of SKP1 homolog AB −2.33* −1.1 REP REP REP REP gi|71895155 20497.5 6.35
6307 mitotic checkpoint protein BUB3 AB −3.53** 1.61 REP REP REP −2.97** gi|57529813 37256.3 6.5
3501 PREDICTED: similar to eukaryotic translation initiation factor 4H isoform 1 1.26 −2.31* −1.33 −1.99 −1.45 −1.84 1.02 gi|126314438 27406.4 6.78
8202 PREDICTED: similar to eukaryotic translation initiation factor 4H isoform 1 1.17 −3.81** 1.13 −2.86** −1.72 −1.38 −2.65** gi|126314438 27406.4 6.78
2013 PREDICTED: similar to histone H2B 3.97* REP REP REP REP REP 8.73** gi|149617840 29704.7 9.68
7117 single-strand binding protein AB AB AB IN IN IN AB gi|42523087 15766.8 6.13
5603 pre-mRNA-processing factor 19 1.61 −1.34 −2.62** −1.23 −1.09 −1.32 −1.16 gi|86129600 55100.2 6.19
Signal transduction
3616 Amb7 1.22 1.9 1.94 4.4** 1.87 IN AB gi|117168610 225516.8 5.61
5518 coronin-1C AB AB AB IN AB AB AB gi|86129440 53174.1 6.22
6102 coronin-1C −1.1 1.32 −1.33 2.41** 3.78* 3.37** 3.76** gi|86129440 53174.1 6.22
3015 fatty acid-binding protein, adipocyte −2.05** 2.38* 2.34** 4.91** 4.73** 2.39** 1.11 gi|45383556 14884.6 6.34
3012 fatty acid-binding protein, heart −1.23 −1.49 AB 9.97** 1.5 1.62 1.77 gi|71894843 14806.6 5.92
3709 Lipocalin precursor 1.75 −1.47 1.09 4.11** 6.62** 3.26* 1.47 gi|225716896 20257.1 7.6
6213 PREDICTED: similar to GADS protein −1.46 −1.24 1.56 −2.49** 1.7 −1.08 1.19 gi|118082689 33178.5 6.23
5205 PREDICTED: similar to GADS protein −1.11 −2.14** −1.15 −3.84** 1.04 −1.16 −1.45 gi|118082689 33178.5 6.23
6310 PREDICTED: similar to nucleic acid binding protein isoform 2 1.42 −1.6 −1.03 −1.41 −2.07** −1.59 −1.63 gi|126345445 35784.3 7
6011 PREDICTED: similar to retinoid binding protein 7 −4.75** IN IN IN IN 4.34** AB gi|118101075 14701.2 5.31
1008 thioredoxin −1.26 −1.11 −1.02 2.46* 1.28 1.7 1.07 gi|45382053 11692.8 5.1
Transport
6517 ferritin heavy chain −1.14 2.81** −1.44 2.13** 4.11** 2.25** 1.02 gi|45384172 21078.3 5.78
7203 ferritin heavy chain −4.57** 1.42 −1.18 3.89** 2.12** 2.49** 1.03 gi|45384172 21078.3 5.78
8018 hemoglobin alpha-A chain −3.13** 1.13 1.41 1.37 1.2 1.4 1.26 gi|4894665 10914.7 7.19
8003 hemoglobin alpha-A chain −2.54** −1.15 −1.12 −1.03 1.28 −1.14 1.06 gi|4894665 10914.7 7.19
6009 hemoglobin subunit alpha-D −1.08 −1.07 3.59** 1.2 1.02 1.04 1.03 gi|52138645 15685 7.01
6804 ovotransferrin BB type 1.03 1.32 −1.27 2.71* 1.82 1.78 1.03 gi|71274075 77781.4 6.85
6810 ovotransferrin BB type AB 1.95 REP 20.2* IN IN AB gi|71274075 77781.4 6.85
6801 ovotransferrin CC type 1.56 1.27 1.19 2.52** 2.89* 2.48* −1.17 gi|71274077 77750.4 7.08
7201 mediator of cell motility 1 1.37 −1.06 1.02 −2.93** −1.09 1.54 −1.24 gi|300676818 31517.5 6.49
Others proteins
6104 603815993F1 CSEQCHN52 Gallus gallus cDNA clone ChEST809j23 5′, mRNA sequence 1.14 −1.17 −1.57 −2.53** −2.12** −1.94 1.19 gi|25541234 23338.9 6.03
304 alpha-tropomyosin (partial) −1.32 1.18 1.12 2.37** 2.38** 2.46** 1.71 gi|212815 30045.3 4.64
5815 conalbumin −1.17 1.11 −1.05 5.97* 3.44** 1.9 −1.12 gi|295721 79551.1 6.85
4012 cytoChrome c subfamily, putative AB IN AB IN IN 26.4 AB gi|124005227 24128.2 9.21
307 putative nucleophosmin 1 variant 1 1.2 −2.66* 1.23 −2.09** −3.13** −1.19 1.06 gi|45383996 32612 4.66
4412 putative protein product of Nbla10058 AB IN AB AB AB AB AB gi|76879893 48587.2 5.85
204 PREDICTED: similar to Acidic leucine-rich nuclear 1.3 −1.71 −1.6 −2.22* −3.2** −2.45** −1.14 gi|118096008 32075.9 3.98
4420 PREDICTED: similar to cyclophilin AB AB AB IN IN AB AB gi|118089782 39402.9 5.61
6304 PREDICTED: similar to KIAA0089 1.01 −1.19 −1.45 −1.43 −2.6** −1.8 1.2 gi|50732786 38564.1 6.55
4116 PREDICTED: similar to LOC129607 protein −1.25 IN AB IN IN IN AB gi|118088982 31865.5 8.45
4114 PREDICTED: similar to MGC84496 protein −1.43 −1.32 −1.37 1.17 1.64 1.98 2.77** gi|118101652 22693.7 6.07
8002 protein S100-A10 −1.27 1.37 1.11 3.15* 1.27 1.3 1 gi|45382861 11282.5 6.82
1006 transthyretin precursor −1.12 −1.01 1.35 −2.64** −3.31** −1.57 −1.09 gi|45384444 16299.1 5.11
Hypothetical proteins
3507 PREDICTED: hypothetical protein 1.52 −1.52 −1.24 −3.08** −2.04* −1.49 −1.31 gi|50762370 47124.5 5.64
2203 PREDICTED: hypothetical protein −1.37 −1.72 −1.72 −6.61** −2.27** −3.83** −1.02 gi|118087111 30122.5 5.51
2209 PREDICTED: hypothetical protein −1.12 −1.22 −2.19** −1.3 −1.27 1.14 1.43 gi|118092623 32616.1 5.53
3110 PREDICTED: hypothetical protein AB −2.73** IN AB −3.04** −2* −1.22 gi|118084734 18881.6 6.08
5108 PREDICTED: hypothetical protein −5.55** REP REP −1.2 IN REP 1.75 gi|118084734 18881.6 6.08
7214 PREDICTED: hypothetical protein −1.48 1.03 −2.13** −1.46 1.88 1.06 −1.04 gi|50762370 47124.5 5.64
2307 hypothetical protein −1.24 1.82 1.47 1.39 1.55 2.28* 2.19** gi|53126859 42107.9 5.31
4013 hypothetical protein Bsuib36_09931 AB AB AB IN AB AB AB gi|254704229 122393.8 8.07
3210 hypothetical protein PANDA_003704 AB AB AB IN AB AB AB gi|281343027 31307.8 5.44
  1. Fold change = infected/control. Positive indicates up regulation, negative indicates down-regulation.
  2. IN = induced, detected only in infected group, REP = repressed, detected only in the control group, AB = absent in both groups, dpi = days post-infection.
  3. Asterisks (*) indicates statistically significant difference. (*) indicates p<0.05 and (**) indicates p<0.01.
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