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Figure 1 | Virology Journal

Figure 1

From: An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting

Figure 1

A) Schematic diagram of constructed recombinant RSV ( rRSV-X) expressing EGFP gene in the first (E1-rRSV-X) and the seventh (E7-rRSV-X) position in the virus genome and the positions of the restriction enzymes in the RSV- X genome used for insertion of EGFP. Blue: RSV gene; green: EGFP gene. B) Replication kinetics of recombinant RSV viruses E1-rRSV-X, E7-rRSV-X and the parental rRSV-X in Vero and Hep-2 cells. Cells were infected at a multiplicity of infection (MOI) of 0.1 with the rRSVs. At the indicated time points, cells and supernatants were collected and virus titers were determined. Closed lines represent growth on Vero cells; dashed lines represent growth on Hep-2 cells.

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