Table 1 Receptor specificity determination assay results of five subtypes of avian influenza viruses with and without α 2, 3-sialidase-enzyme treatment of RBCs
No. | Virus strain | HA titer with enzyme treated 1% goose RBCs | HA titer with untreated 1% goose RBCs | HA titer with enzyme treated 0.5% turkey RBCs | HA titer with untreated 0.5% turkey RBCs | Receptor specificity Avian / mammalian | Amino acid present at position 221 |
|---|---|---|---|---|---|---|---|
1 | A/chicken/India/NIV33487/06-RG-2008 (H5N1) | No titer | 512 | No titer | 512 | SA α 2,3 Gal –Avian | S |
2 | A/chicken/India/WB-NIV2665/2008 (H5N1) | 16 | 16 | 16 | 16 | SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian | P |
3 | A/chicken/India/WB-NIV2670/2008 (H5N1) | No titer | 32 | No titer | 128 | SA α 2,3 Gal–Avian | P |
4 | A/chicken/Manipur/NIV9743/2007 (H5N1) | No titer | 8 | No titer | 24 | SA α 2,3 Gal–Avian | S |
5 | A/chicken/India/WB-NIV2811/2008 (H5N1) | No titer | 24 | No titer | 256 | SA α 2,3 Gal–Avian | S |
6 | A/chicken/India/AS-NIV15983/2008 (H5N1) | 8 | 192 | 16 | 512 | SA α 2,3 Gal and α 2, 6 Gal-Avian and Mammalian | S |
7 | A/duck/India/TR-NIV4396/2008 (H5N1) | No titer | 32 | No titer | 24 | SA α 2,3 Gal–Avian | S |
8 | A/chicken/India-WB-NIV82853/2008 (H5N1) | No titer | 16 | No titer | 768 | SA α 2,3 Gal–Avian | S |
9 | A/chicken/India/AS-NIV15618/2008 (H5N1) | No titer | 48 | No titer | 32 | SA α 2,3 Gal–Avian | S |
10 | A/chicken/India/AS-NIV16717/2008 (H5N1) | No titer | 16 | No titer | 16 | SA α 2,3 Gal–Avian | S |
11 | A/chicken/Pune/099321/2009 (H9N2) | 512 | 1024 | 512 | 1024 | SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian | P |
12 | A/aquatic bird/India/NIV-17095/2007 (H11N1) | 1024 | 1024 | 512 | 1024 | SA α 2,3 Gal and α 2, 6 Gal- Avian and Mammalian | P |
13 | A/Duck/Nabagram-WB/101018/2009 (H4N6) | No titer | 512 | No titer | 128 | SA α 2,3 Gal–Avian | P |
14 | A/chicken/Italy/1067/1999 (H7N1) | No titer | 512 | No titer | 512 | SA α 2,3 Gal–Avian | P |