Table 4 Strains and plasmids used in this study
Strain | Description | Source/Reference |
|---|---|---|
Escherichia coli SM10 | - | [39] |
Bacillus anthracis 34F2 | Sterne pXO1+pXO2- | Hanna lab |
Bacillus anthracis BA663 | 7702 Sterne pXO1+pXO2- | Koehler lab |
Bacillus anthracis JB220 | 7702 ΔBAS2245 | Calendar lab |
Bacillus anthracis S-R1 through S-R9 | Spontaneous AP50R mutants | This study |
Bacillus anthracis J-R1 through J-R8 | Spontaneous AP50R mutants | This study |
Bacillus anthracis BAP350 | BA663 ΔcsaB | This study |
Bacillus anthracis BAP356 | BA663(AP50R-3)-csaB ∇G234 | This study |
Bacillus anthracis BAP366 | BA663(AP50R-1)-csaB ∇G46 | This study |
Bacillus anthracis BAP411 | BA663 (AP50R-6)-csaB ∇GCTTA219 | This study |
Bacillus anthracis BAP435 | BA663 (AP50R-4)-csaB ΔA203 | This study |
Bacillus anthracis BAP503 | BA663 csaB H270A | This study |
Bacillus anthracis BAP533 | BAP350 csaB A693C creating an XmaI site | This study |
Bacillus anthracis BAP553 | BA663 ΔBAS3946 | This study |
Bacillus anthracis BAP560 | BA663 with BAS 3946 G1024A mutation | This study |
Bacillus anthracis BGBN 001 | BAP350/pBGBN1003 | This study |
Plasmid pRP1028 | Mutant construction | This study |
Plasmid pSS4332 | Mutant construction | This study |
Plasmid pSW4 | E. coli- B. anthracis shuttle vector | [37] |
Plasmid pBGBN1001 | pSW4 NotI | This study |
Plasmid pBGBN1002 | pSW4 oriT | This study |
Plasmid pBGBN1003 | pSW4 oriT csaB | This study |
Plasmid pGOv4 | pUC19 AmpR, KanR | Gene Oracle |
Plasmid pNV36 | pGOv4 ΔBAS3946 | Gene Oracle |
Plasmid pNV37 | pGOv4 BAS3946 G1024A | Gene Oracle |