Whole genome sequencing of phage resistant Bacillus anthracismutants reveals an essential role for cell surface anchoring protein CsaB in phage AP50c adsorption

Table 2 Location of true positive variations in genomes of mutant B. anthracis compared to reference genome

Strain	Start position	Stop position	Reference allele	Sample allele	Locus description	Coverage	Percent Concordance
S-R1	894,910	894,910	-	G	CsaB protein	10	100
	3,894,626	3,894,626	G	A	2,3-diketo-5-methylthiopentyl-1-phosphate enolase	8	100
S-R2	210,679	210,679	T	G	intergenic	29	97
	895,097	895,097	-	G	CsaB protein	38	82
	2,139,026	2,139,026	G	T	sensor histidine kinase	34	76
	3,894,626	3,894,626	G	A	2,3-diketo-5-methylthiopentyl-1-phosphate enolase	24	100
S-R3	895,097	895,097	-	G	CsaB protein	23	100
	3,894,626	3,894,626	G	A	2,3-diketo-5-methylthiopentyl-1-phosphate enolase	19	100
S-R4	895,066	895,066	A	-	CsaB protein	18	100
	3,894,626	3,894,626	G	A	2,3-diketo-5-methylthiopentyl-1-phosphate enolase	19	100
S-R6	160,743	160,743	A	-	pX01	58	86
	895,083	895,083	-	GCTTA	CsaB protein	17	88
	1,330,746	1,330,746	G	CGGT	intergenic	4	75
	3,894,626	3,894,626	G	A	2,3-diketo-5-methylthiopentyl-1-phosphate enolase	14	100
	4,303,652	4,303,652	-	AC	solute-binding family 5 protein	20	100
J-R1	68,981	68,981	A	-	hypoxanthine-guanine phosphoribosyltransferase	33	97
	895,436	895,436	G	T	CsaB protein	23	96

ISSN: 1743-422X