A divergent variant of Grapevine leafroll-associated virus 3is present in California

Table 1 Primers used in the amplification of the CA7246 genome, with locations referring to the 5' nucleotide, relative to CA7246's genome sequence

Direction	Location	Name	Sequence(5’ → 3’)	Used for
R	312	LR3E_FG300RACE	CAACACTACGCGCAAGAAAAGAGC	5' RACE
R	3254	LR3E_FG3258R	CGCTTGAAAGAACAGCCTGAAGATGTTC	RT, PCR
R	8193	LR3E_FG8194R	AGTGTCCATCCCATGGTAGAACAACCA	RT, PCR
R	11733	RT_FG11884	ACGTCTTTACGCACTTTCGAGAGA	RT, PCR
R	13357	LR3E-RDRP-R	AATTTCTCTGCGAGCTCAGGGCA	RT, PCR
R	14079	St E 13988-R	TACCACCGGTATGGTCGCCAGT	RT, PCR
R	14397	CP-580R	GCCCATAACCTTCTTACACA	RT, PCR
R	17805	St E 17713-R	CCCTCTTTCCACGACACACTTCG	RT, PCR
R	18443	LR3E_FG18376Rb	TATCACTATCGACTTTACGGACTAAT	RT, PCR
F	5	LR3E_FG5	ATGCTCTAGTAGGATTCGAACACGGCA	PCR
F	2973	LR3E_FG2976F	GTCGACAGGATCTATTCCTACGCGC	PCR
F	8045	LR3E_FG8045F	CACCTTTGTCGAACTACGTCACAGGG	PCR
F	10786	LR3E-RDRPFN-1	GGGGATAGCCGGATGTACACCGG	PCR
F	11335	LR3E-RDRPFN-2	TTTCGACGTCTCCTTCGTGAAG	PCR
F	13239	LR3E - Minus 710	TATGTACCAATCGAGTCGTTCG	PCR
F	13927	CP-130 F	GAACTGAAATTAGGGCAGATATA	PCR
F	14058	St E 13988-F	ACTGGCGACCATACCGGTGGTA	PCR
F	18044	3RACE_FG17991F	GCGATCGCTACTATAGTCGTGGTGA	3' RACE

Location refers to the 5’ nucleotide, relative to CA7246’s genome sequence, JQ796828. Reverse primers were used for both RT-PCR and PCR.

ISSN: 1743-422X