Table 1 Sequences of primers used for the PCRs
Primers | Sequences (5'-3') | Underlined restriction site | Position on MYVB |
|---|---|---|---|
Y47βp138-F | GAATTCAAGCTTCTCCATCATATCTTTAAAGT | EcoRI, HindIII | 690-671 |
Y47βp214-F | GAATTCAAGCTTCTCATGTGTACGTATATATACG | EcoRI, HindIII | 766-745 |
Y47βp267-F | GAATTCAAGCTTCGTTTTCTTTCCTTTTCAGTTCAG | EcoRI, HindIII | 819-796 |
Y47βp389-F | GAATTCAAGCTTCAAACGACATCGTTTTCGAGTT | EcoRI, HindIII | 941-920 |
Y47βp418-F | GAATTCAAGCTTTTATTATTTTTTTCTTTTTTTCTTC | EcoRI, HindIII | 971-947 |
Y47βp-F | GAATTCAAGCTTTTTCAAATAACAGATCATCAAG | EcoRI, HindIII | 195-174 |
Y47βp-R | GAGCTCGGATCCCTTGCTTGTGTGATGGAAGTG | SacI, BamHI | 553-573 |
Y47βΔUTR-F | TTTTCTTTTTCTCAAACGACATCGTTTTCG | – | 962-953 plus 943-924 |
Y47βΔUTR-R | GTCGTTTGAGAAAAAGAAAAAAATAATAATGGGG | – | 934-943 plus 953-976 |
β01 | GTAGGTACCACTACGCTACGCAGCAGCC | KpnI | 1290-1308 |
β02 | AGTGGTACCTACCCTCCCAGGGGTACAC | KpnI | 1283-1265 |
β03 | GTAGAAACCACTACGCTACGCAGCAGCC | – | 1290-1308 |