Increased proportions of Th22, Th17 and Th1 cells in AVMC were investigated by flow cytometry analysis, on day 14 post injection. A.Th22 cells within CD4+T cells were identified based on their expressions of IL-22+IL-17-IFN-γ-CD4+. B.Th17 and Th1 cells within CD4+T cells were determined based on their expressions of IL17+CD4+ and IFN-γ+CD4+. C. Comparisons of Th22, Th17 and Th1 cells in AVMC and control groups. D. The correlation analysis of Th22 and Th17, Th22 and Th1 cells in AVMC group. Each point represented an individual mouse. **p < 0.01. Data are mean ± SD.