18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells

Table 2 Bestkeeper correlation and regression analysis of housekeeping genes

	18S rRNA	GAPDH	ACTB	ATP5B/ ATP5G1cp
	vs.	vs.	vs.	vs.
	BestKeeper	BestKeeper	BestKeeper	BestKeeper
	Coefficient of correlation [r]
HBECs	0.962	0.871	0.709	0.543
PTECs	0.843	0.743	0.741	0.73
Chicken lung cells	0.944	0.832	0.845	-
Duck lung cells	0.836	0.792	0.542	-

Pairwise correlation and regression analysis using Bestkeeper correlation analysis (n=12) found 18S rRNA as the most suitable housekeeping gene. Note that highest correlation between 18S rRNA and the BestKeeper index (p<0.01) was found in all the four cell types used. *ATP5B and ATP5G1were included in HBECs and PTECs analysis respectively.

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