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Table 2 Bestkeeper correlation and regression analysis of housekeeping genes

From: 18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells

  18S rRNA GAPDH ACTB ATP5B/ ATP5G1cp
  vs. vs. vs. vs.
  BestKeeper BestKeeper BestKeeper BestKeeper
  Coefficient of correlation [r]
HBECs 0.962 0.871 0.709 0.543
PTECs 0.843 0.743 0.741 0.73
Chicken lung cells 0.944 0.832 0.845 -
Duck lung cells 0.836 0.792 0.542 -
  1. Pairwise correlation and regression analysis using Bestkeeper correlation analysis (n=12) found 18S rRNA as the most suitable housekeeping gene. Note that highest correlation between 18S rRNA and the BestKeeper index (p<0.01) was found in all the four cell types used. *ATP5B and ATP5G1were included in HBECs and PTECs analysis respectively.