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Table 1 Bestkeeper analysis of housekeeping genes showing variation in gene expression

From: 18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells

  Standard deviation [± Cp]
HBECs 18S rRNA ACTB GAPDH ATP5B / ATP5G1 *
H5N1 tyTR05 0.474 2.484 1.196 1.620
Swine H1N1 0.375 0.484 0.465 0.413
USSR huH1N1 0.462 1.046 0.740 0.662
Average 0.437 1.338 0.800 0.899
PTECs     
Swine H1N1 0.265 0.354 0.344 0.413
H5N1 tyEng91 0.090 0.330 0.310 0.140
H5N1 tyTR05 0.100 0.420 0.240 0.450
Average 0.152 0.368 0.298 0.334
Chicken lung cells     
H5N1 tyEng91 0.160 0.510 0.480 -
H5N1 tyTR05 0.140 0.230 0.260 -
Avian H2N3 0.200 0.230 0.200 -
Average 0.167 0.323 0.313 -
Duck lung cells     
H5N1 tyEng91 0.380 0.480 0.690 -
H5N1 tyTR05 0.160 0.660 1.290 -
Avian H2N3 0.110 0.190 0.430 -
Average 0.217 0.443 0.803 -
  1. Bestkeeper analysis (n=6) of standard deviation (± Cp) showed 18S rRNA is the most stable gene in all the four cell types at 24h post-infection with the influenza virus subtypes used in this study. *ATP5B and ATP5G1 were included in HBECs and PTECs analysis respectively.