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Table 1 Bestkeeper analysis of housekeeping genes showing variation in gene expression

From: 18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells

 

Standard deviation [± Cp]

HBECs

18S rRNA

ACTB

GAPDH

ATP5B / ATP5G1 *

H5N1 tyTR05

0.474

2.484

1.196

1.620

Swine H1N1

0.375

0.484

0.465

0.413

USSR huH1N1

0.462

1.046

0.740

0.662

Average

0.437

1.338

0.800

0.899

PTECs

    

Swine H1N1

0.265

0.354

0.344

0.413

H5N1 tyEng91

0.090

0.330

0.310

0.140

H5N1 tyTR05

0.100

0.420

0.240

0.450

Average

0.152

0.368

0.298

0.334

Chicken lung cells

    

H5N1 tyEng91

0.160

0.510

0.480

-

H5N1 tyTR05

0.140

0.230

0.260

-

Avian H2N3

0.200

0.230

0.200

-

Average

0.167

0.323

0.313

-

Duck lung cells

    

H5N1 tyEng91

0.380

0.480

0.690

-

H5N1 tyTR05

0.160

0.660

1.290

-

Avian H2N3

0.110

0.190

0.430

-

Average

0.217

0.443

0.803

-

  1. Bestkeeper analysis (n=6) of standard deviation (± Cp) showed 18S rRNA is the most stable gene in all the four cell types at 24h post-infection with the influenza virus subtypes used in this study. *ATP5B and ATP5G1 were included in HBECs and PTECs analysis respectively.
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Virology Journal

ISSN: 1743-422X