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Table 1 Cell lines

From: Infectious pancreatic necrosis virus (IPNV) from salmonid fish enters, but does not replicate in, mammalian cells

Cell lines

Growth media(*)

Chinook salmon embryo cells (CHSE)-214 (Fryer et al., 1965)

Ørpetveit et al., 2008

Rabbit kidney (RK)-13 cells (ATCC CCL-37)

MEM Eagle’s with Earle’s BSS (EMEM) supplemented with 10% FBS, 4 mM L-glutamine and 50 μg/ml gentamicin, final pH 7.6

Foetal bovine turbinate (FBT) cells (“in house” cell line)

As described for RK-13 cells, but supplemented with 16.4 mM Tris-buffer (pH 7,8) and 5.3 mM NaHCO3, final pH 7.6

Vero cells ATCC CCL-81TM

RPMI-1640 (Sigma-Aldrich, St Louis, MO) supplemented with 10% FBS and 50 μg/ml gentamicin.

Primary human embryonic fibroblast (HE) cells (a kind gift from Rikshospitalet University Hospital, Oslo, Norway)

 

A549 cells (human) (ATCC CCL-185TM)

 

THP-1 cells (human) (ATCC TIB-202TM)

 
  1. (*) Where not otherwise indicated, the cell culture reagents were obtained from Cambrex Bio Science, Rockland, ME.