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Figure 3 | Virology Journal

Figure 3

From: Generation and characterization of a recombinant Newcastle disease virus expressing the red fluorescent protein for use in co-infection studies

Figure 3

Co-infection of DF-1 with rLS-RFP and rB1-GFP. (A) Monolayers of DF-1 cells were infected with rLS-RFP and rB1-GFP (MOI=3) at the same time point. At 24h post infection, cells were stained with HCS NuclearMask Blue and examined under a fluorescence microscope. a. merged green, red and blue channels; b. red channel; c. green channel, d. merged green and red channels; e. bright field. (B) Percentage of infected cells. Cells were infected as described in A. Three fields were randomly selected and used to determine the numbers of red-, green- and yellow-fluorescent cells using the ImageJ software. Results shown represent mean percentages of three fields. The percentages of cells infected with each virus (rLS-RFP of rB1-GFP) and the percentage of cells co-infected are expressed as a relative percentage of the total number of infected cells.

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Virology Journal

ISSN: 1743-422X