Figure 2

Key signal transduction pathfinder genes altered after ATIII treatment of HCV OR6 replicon. Analysis of activated signal transduction pathways in HCV OR6 replicon cells after treatment with ATIII. 5x10⁴ HCV OR6 replicon cells were treated with 7, 17, 58 μM ATIII for 48 h. A real-time PCR expression array was used to analyze the expression of 84 key genes from 18 signal transduction pathways. Asterisks show genes with significant changes (*, P<0,05; **, P<0.01; *** P<0.001) in gene expression compared to vehicle control as calculated by the ΔΔC_t method of three independent experiments are shown. All genes are normalized using the GAPDH house-keeping gene. Only genes with more than a 5-fold change in gene-expression are shown.