Figure 2

Representative agarose gel of the nested polymerase chain reaction (PCR) produts. Electrophoresis and subsequent ethidium bromide staining of amplicons from the nested PCR amplification with pol-IN subclade F1-specific primers of the DNA samples known to harbor HIV-1 subclade F1 (A), and subtype B (B). Amplicons of nested PCR (1247 bp) performed with L-pol subclade F1-specific primers using samples that were observed to be infected with HIV-1 subclade F1 (solid line), BF1 recombinants with breakpoints located between the PCR primers (dotted line), and subtype B (dashed line). (C). Amplicons of nested PCR (727 bp) performed with M-pol primers (specific for F1 and BF1 variants) using samples observed to be infected with HIV-1 subclade F1 (solid line), BF1 recombinants with breakpoints located between the PCR primers (dotted line), and subtype B (dashed line) (D). M, molecular weight DNA marker (100 bp DNA ladder, Invitrogen).