Skip to main content

Advertisement

Table 2 ND10 proteins found in association with transfected or integrated operator

From: Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression

Cell type Operator/repressor PML SUMO-1 Daxx Sp100 NDP55 HP1
HEp-2 cotransfected + + + + + -
HEp-2 only operator transfected (FISH) - - - - - -
MEF PML−/− cotransfected 0 + + + +/− -
MEF Daxx−/− cotransfected + + 0 + + -
293 Sp100 - cotransfected + + + 0 + -
HEp-2-Op256 operator integrated, repressor transfected + + + + + -
  1. Various cell lines, some missing specific ND10-associated proteins (0), were co-transfected with plasmids carrying either the lac operator repeats or the GFP-repressor protein and evaluated by fluorescent microscopy to determine whether specified ND10-associated proteins colocalized with (+) or were present apart from the operator GFP-repressor complex (−). For a quantitative assessment of various integrated operator sequences, see also Table 1. Repeats in the Presence or Absence of GFP-repressor Protein.