Nuclear domain 10-associated proteins recognize and segregate intranuclear DNA/protein complexes to negate gene expression

Table 2 ND10 proteins found in association with transfected or integrated operator

Cell type	Operator/repressor	PML	SUMO-1	Daxx	Sp100	NDP55	HP1
HEp-2	cotransfected	+	+	+	+	+	-
HEp-2	only operator transfected (FISH)	-	-	-	-	-	-
MEF PML−/−	cotransfected	0	+	+	+	+/−	-
MEF Daxx−/−	cotransfected	+	+	0	+	+	-
293 Sp100 -	cotransfected	+	+	+	0	+	-
HEp-2-Op256	operator integrated, repressor transfected	+	+	+	+	+	-

Various cell lines, some missing specific ND10-associated proteins (0), were co-transfected with plasmids carrying either the lac operator repeats or the GFP-repressor protein and evaluated by fluorescent microscopy to determine whether specified ND10-associated proteins colocalized with (+) or were present apart from the operator GFP-repressor complex (−). For a quantitative assessment of various integrated operator sequences, see also Table 1. Repeats in the Presence or Absence of GFP-repressor Protein.

ISSN: 1743-422X