Cell type
|
Operator/repressor
|
PML
|
SUMO-1
|
Daxx
|
Sp100
|
NDP55
|
HP1
|
---|
HEp-2
|
cotransfected
|
+
|
+
|
+
|
+
|
+
|
-
|
HEp-2
|
only operator transfected (FISH)
|
-
|
-
|
-
|
-
|
-
|
-
|
MEF PML−/−
|
cotransfected
|
0
|
+
|
+
|
+
|
+/−
|
-
|
MEF Daxx−/−
|
cotransfected
|
+
|
+
|
0
|
+
|
+
|
-
|
293 Sp100 -
|
cotransfected
|
+
|
+
|
+
|
0
|
+
|
-
|
HEp-2-Op256
|
operator integrated, repressor transfected
|
+
|
+
|
+
|
+
|
+
|
-
|
- Various cell lines, some missing specific ND10-associated proteins (0), were co-transfected with plasmids carrying either the lac operator repeats or the GFP-repressor protein and evaluated by fluorescent microscopy to determine whether specified ND10-associated proteins colocalized with (+) or were present apart from the operator GFP-repressor complex (−). For a quantitative assessment of various integrated operator sequences, see also Table 1. Repeats in the Presence or Absence of GFP-repressor Protein.