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Table 2 Pairwise comparison of LR3.HRM4 amplicon (226 nt segment of Hsp70h) for each variant group

From: Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

   Variant group representative isolates
   1 2 3 4 5 6 7 8 9 10
Group I_GU983863.1_GLRaV-3_Isolate_WA-MR 1   0 1 1 9 10 24 50 50 53
Group I_AF037268.2_GLRaV-3_Isolate_NY-1 2 100   1 1 9 10 24 50 50 53
Group I_EU344893.1_GLRaV-3_Isolate_Cl-766 3 99.56 99.56   2 9 10 24 50 50 53
Group I_GQ352631.1_GLRaV-3_Isolate_621 4 99.56 99.56 99.12   10 11 25 49 49 52
Group II_GQ352632.1_GLRaV-3_Isolate_623 5 96.02 96.02 96.02 95.58   2 27 49 49 53
Group II_EU259806.1_GLRaV-3_Isolate_GP18 6 95.58 95.58 95.58 95.13 99.12   27 51 51 53
Group III_GQ352633.1_GLRaV-3_Isolate PL-20 7 89.38 89.38 89.38 88.94 88.05 88.05   58 58 61
Group VI_JQ655295_GLRaV-3_Isolate GH11 8 77.88 77.88 77.88 78.32 78.32 77.43 74.34   0 10
Group VI_JQ655296_GLRaV-3_Isolate_GH30 9 77.88 77.88 77.88 78.32 78.32 77.43 74.34 100   10
Group VI_EF508151.1_GLRaV-3_Isolate_NZ-1 10 76.55 76.55 76.55 76.99 76.55 76.55 73.01 95.58 95.58  
  1. The upper comparison in bold is the number of nucleotide differences between variant sequences and the lower comparison is the percent identity (%) between variant sequences.