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Table 1 Descriptive statistics of melting points generated by real-time RT-PCR HRM assays with primer pairs LR3.HRM4 and LR3.HRM6

From: Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

Variant group Number of data pointsa Min Max Mean Temperature range between upper and lower limit
LR3.HRM4      
  Group I 31 83.20 83.98 83.60 0.78
  Group II 203 83.15 84.25 83.77 1.10
  Group III 73 84.87 85.70 85.44 0.83
  Group VI 142 85.30 86.37 85.97 1.07
LR3.HRM6      
  Group I 27 84.78 85.42 85.03 0.64
  Group II 187 85.95 86.90 86.41 0.95
  1. aNumber of melting point temperatures generated per variant group from the 121 samples. More than one melting point temperature per sample was generated due to mix infections and duplex reactions.