Real-time RT-PCR high-resolution melting curve analysis and multiplex RT-PCR to detect and differentiate grapevine leafroll-associated virus 3 variant groups I, II, III and VI

Table 1 Descriptive statistics of melting points generated by real-time RT-PCR HRM assays with primer pairs LR3.HRM4 and LR3.HRM6

Variant group	Number of data points^a	Min	Max	Mean	Temperature range between upper and lower limit
LR3.HRM4
Group I	31	83.20	83.98	83.60	0.78
Group II	203	83.15	84.25	83.77	1.10
Group III	73	84.87	85.70	85.44	0.83
Group VI	142	85.30	86.37	85.97	1.07
LR3.HRM6
Group I	27	84.78	85.42	85.03	0.64
Group II	187	85.95	86.90	86.41	0.95

^aNumber of melting point temperatures generated per variant group from the 121 samples. More than one melting point temperature per sample was generated due to mix infections and duplex reactions.

ISSN: 1743-422X