Epitope mapping by random peptide phage display reveals essential residues for vaccinia extracellular enveloped virion spread

Table 1 Primers used for recombinant A33 construction by site-directed mutagenesis of the A33R ^VACV gene

Mutation	Primer sequence^a
D115A	ACATTCAGCATACCAGTTATTCTCGGATGCTAAAGCAAATTGCACTGCGG
Y116A	ACATTCAGACGCACAGTTATTCTCGGATGCTAAAGCAAATTGCACTGCGG
Q117A	ACATTCAGACTACGCATTATTCTCGGATGCTAAAGCAAATTGCACTGCGG
L118A	ACATTCAGACTACCAGGCATTCTCGGATGCTAAAGCAAATTGCACTGCGG
S120A	ACATTCAGATTACCAGTTATTCGCTGATGCTAAAGCAAATTGCACTGCGG
N125A	ACATTCAGACTACCAGTTATTCTCGGATGCTAAAGCAGCATGCACTGCGG
E129A	ACATTCAGACTACCAGTTATTCTCGGATGCTAAAGCAAATTGCACTGCGGCATCATCAAC
D115A,Q117A	ACATTCAGCTTACGCATTATTCTCGGATGCTAAAGCAAATTGCACTGCGG
D115A,N125A	ACATTCAGCTTACCAGTTATTCTCGGATGCTAAAGCAGCATGCACTGCGG
Q117A,N125A	ACATTCAGACTACGCATTATTCTCGGATGCTAAAGCAGCATGCACTGCGGAATCATCAAC
L118A,E129A	ACATTCAGACTACCAGGCATTCTCGGATGCTAAAGCAAATTGCACTGCGGCATCATCAAC

^aAll primers listed in 5′-3′ direction with the position of the mutation(s) underlined. A common reverse primer was used for all reactions:
AATATATAACAAGAACCCTGGTAATAGAGACCATTACAGCTTTC.

ISSN: 1743-422X