SARS-CoV S-specific humoral immune responses in the immunized mice. Mice were immunized with rADV-S by s.l., i.n., or i.m. route. Sera were collected two weeks after each vaccination and BAL was collected two weeks after the last immunization. S-specific IgG titers in sera (A) and S-specific IgA titers in BAL (B) were determined by ELISA. The results are expressed as the means + SD. The data are representative of three separate experiments.