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Table 2 Primers for real-time PCR

From: Strong innate immune response and cell death in chicken splenocytes infected with genotype VIId Newcastle disease virus

Target RNA

Primer sequence (5’-3’)

Accession no.

Product size (bp)

IFN-α

Forward primer: CCACGACATCCTTCAGCACCT Reverse primer: TGAGGAGGCTTTGGCGTTG

NM_205427.1

89

IFN-β

Forward primer: TGCACAGCATCCTACTGCTCTTG Reverse primer: GTTGGCATCCTGGTGACGAA

NM_001024836.1

83

IFN-γ

Forward primer: AGCATTTGAACTGAGCCATCACC Reverse primer: CGTCAGCTACATCTGAATGACTTG

NM_205149.1

181

β-actin

Forward primer: ATTGTCCACCGCAAATGCTTC Reverse primer: AAATAAAGCCATGCCAATCTCGTC

NM_205518.1

113

M

Forward primer: GCTTGTGAAGGCGAGAGGTG Reverse primer: AACCTGGGGAGAGGCATTTG

-- a

99

  1. a: primers for viral M gene were designed based on the results of sequence comparison as described in the Methods.
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Virology Journal

ISSN: 1743-422X