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Table 1 E. coli strains used to assess the virulence of the three isolates of the rogue phage as determined by the microplate virulence assay

From: Endemic bacteriophages: a cautionary tale for evaluation of bacteriophage therapy and other interventions for infection control in animals

Strain No. Serotype E. coliO157 Phage Type Strain description Minimum Phage MOI1
LRCSh151d1 LRCSh158d1 LRCSh166d1
EC19990293 O157:H7 PT21 Phage type reference strain 0.00001 0.000001 0.000001
EC19990295 O157:H7 PT4 Phage type reference strain 0.00001 0.00001 0.000001
EC19990296 O157:H7 PT23 Phage type reference strain 0.000001 0.000001 0.00001
EC19990298 O157:H7 PT14 Phage type reference strain 0.00001 0.000001 0.000001
EC19990299 O157:H7 PT14 Phage type reference strain 0.01 0.00001 0.001
EC19990300 O157:H7 PT2 Phage type reference strain 0.0001 0.0001 0.00001
EC20010992 O7:H21 NA2 Non-pathogenic E. coli >10 >10 >10
EC19990779 O173:HUN NA Non-pathogenic E. coli >10 >10 >10
EC20030480 O157:H7 PT4 Challenge strain LRC.E318N 0.000001 0.000001 0.000001
EC20030479 O157:H7 PT1 Challenge strain LRC.E319 >10 >10 >10
EC20030478 O157:NM PT87 Challenge Strain LRC.E32511N 10 10 10
EC20030477 O157:H7 PT87 Challenge Strain LRC.H4420nal 0.000001 0.00001 0.000001
EC20030481 O157:H7 PT14 Challenge Strain LRC.R508N 0.00001 0.00001 0.000001
EC19940312 O157:H7 PT8 Challenge Strain LCDC.CO281–31nal 0.000001 0.0001 0.01
  1. 1 MOI: Multiplicity of Infection required for complete lysis of 1 CFU of the tested strains by the listed phages. Phages were identified as virulent for a bacterial strain when the MOI was ≤10. 2 NA: not applicable. Description: Table (Microsoft Word).