Figure 2
From: Sf-PHB2, A new transcription factor, Drives WSSV Ie1Gene Expression via a 12-bp DNA Element
Purification of the 12-p DNA-binding protein using a tandem DNA-affinity columns. (A) Concatamerization of specific DNA oligonucleotides using self-priming PCR. PCR products with the concatamers of the 12-bp oligonucleotide were analyzed using agarose electrophoresis. 1, self-primer PCR products; M, DNA ladder. (B) SDS-PAGE analysis of the purified 12-bp DNA-binding protein. 1, the purified protein; M, protein markers. (C) Identification of the captured proteins using mass spectrometry, two representative peptide mass fingerprint profiles VPWFQYPIIYDIR (A) and FNASQLITQR (B).