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Table 2 The diagram of mouse immunization

From: Multiple linear epitopes (B-cell, CTL and Th) of JEV expressed in recombinant MVA as multiple epitope vaccine induces a protective immune response

Groups Priming Boost 1 Boost 2
rMVA-mep 105TCID50 106 TCID50/0.1 mL 106 TCID50/0.1 mL 106 TCID50/0.1 mL
106 TCID50 107 TCID50/0.1 mL 107 TCID50/0.1 mL 107 TCID50/0.1 mL
107 TCID50 108 TCID50/0.1 mL 108 TCID50/0.1 mL 108 TCID50/0.1mL
Inactivated JEV vaccine 0.1 mL (2x106pfu) 0.1 mL(2x106pfu) 0.1 mL(2x106pfu)
EDIII 50 μg CFA 50 μg IFA 50 μg IFA
rMEP 50 μg CFA 50 μg IFA 50 μg IFA
PBS 0.1 mL 0.1 mL 0.1 mL
Wild MVA 0.1 mL(2x106pfu) 0.1 mL(2x106pfu) 0.1 mL(2x106pfu)
  1. CFA, complete Freund’s adjuvant; IFA, incomplete Freund’s adjuvant.