Specific antibody responses. The sera were collected on day 14 after prime, 1st boost and 2nd boost immunized for detecting the antibody special to rMEP of JEV by ELISA, respectively. The data are shown as mean ± SD of five mice and are fully representative for the individual mice tested. (A) IgG antibody; (B) IgG1 antibody; (C) Ig2a antibody. Statistically significant differences (P < 0.05) are indicated with different small letters.