Identification of rMVA-mep. (A, B, C) Identification of rMVA-mep by PCR using primers targeted to MEP, to k1l and to MVA gene. (A) Lane 1, 2, 3, 4: infected cell focus RK-13-6, RK-13-4, RK-13-3, and RK-13-1, respectively. M: DL2000 marker. (B) Lane 6, 7, 8: infected cell focus RK-13-6(k1l gene), RK-13-6(MEP gene), and RK-6(no wild MVA), respectively. M: DL2000 marker. (C) Lane 10, 11, 12, 13: infected cell focus BHK-21-6, BHK-21-4, BHK-21-2, and BHK-21-1, respectively. M: DL2000 marker. (D) Western Blot analysis of rMVA-mep infected BHK-21. M: Low molecular protein marker; lane 1: Western blot of purified rMEP in E. coli. Lane 2: Western blot of purified rMVA-mep infected BHK-21-6 (The sixth generation on BHK-21 cells). Lane 3: Western blot of purified rMVA-mep infected BHK-21-16. Lane 4: BHK-21 cells.