Construction of the rMVA-mep. A. Design and construction of the multi-epitope peptide (MEP). The MEP was constructed from six B-cell epitopes and two T-cell epitopes, with a glycine and a serine (GS) as a spacer between epitopes. The amino acid sequences of the epitopes were obtained from the envelope protein of the JEV (AF315119) . B. The construction of the rMVA-mep. pGEM-K1L-mep contains the multi-epitope peptide (MEP).