Figure 4

Effect on release of cytochrome c by regulating Bcl-2 family protein in BHV-1-infected cells. (A) Bcl-2 and Bax protein expression in cells mock-infected or BHV-1-infected (2 MOI) for indicated hours (left panel) or different MOI for 48 h (right panel) were analyzed by Western Blot Analysis. (B) qRT-PCR assay for mRNA levels of Bax .Data are mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 versus mock infection. (C) qRT-PCR assay for mRNA levels of Bcl-2. Data are mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 versus mock infection. (D) Cytochrome c release of BHV-1-infected cells. Cells were mock-infected or BHV-1-infected at 2 MOI for different hours, and analyzed by Western Blot Analysis. Cox4 and β-actin were used as internal controls for the mitochondrial fractions and the cytosolic fraction, respectively. Data are representative of three separate experiments. (E) Apaf-1 expression in cells mock-infected or BHV-1-infected (2 MOI) for indicated hours (left panel) or different MOI for 48 h (right panel) were analyzed by Western Blot Analysis.