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Table 4 Epitope mapping of anti-V3 mAbs with consensus-C V3 overlapping peptides

From: Production and characterization of human anti-V3 monoclonal antibodies from the cells of HIV-1 infected Indian donors

 

1Amino acid sequence of C2-V3-C3 overlapping peptides

2anti-V3 mAbs

 

Peptide ID

IIVHLNESVEIV CTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHC NISEEKWNKTLQ

277

903

904

447-52D

1418

9256

IIVHLNESVEIVCTR

>10

>10

>10

>10

>10

9257

LNESVEIVCTRPNNN

>10

>10

>10

>10

>10

9258

VEIVCTRPNNNTRKS

>10

>10

>10

>10

>10

9259

CTRPNNNTRKSIRIG

>10

>10

>10

>10

>10

9260

NNNTRKSIRIGPGQT

>10

>10

>10

0.049

>10

9261

RKSIRIGPGQTFYAT

4.04

1.37

0.327

0.149

>10

9262

RIGPGQTFYATGDII

>10

>10

>10

>10

>10

9263

GQTFYATGDIIGDIR

>10

>10

>10

>10

>10

9264

YATGDIIGDIRQAHC

>10

>10

>10

>10

>10

9265

DIIGDIRQAHCNISE

>10

>10

>10

>10

>10

9266

DIRQAHCNISEEKWN

>10

>10

>10

>10

>10

9267

AHCNISEEKWNKTLQ

>10

>10

>10

>10

>10

  1. 1Amino acid sequences of linear overlapping peptides encompassing the third variable region (V3: Underlined (middle)), flanked by second (C2: (left)) and third (C3: (right)) constant regions and are aligned with the corresponding consensus-C gp120 sequence.
  2. 2Four anti-V3 antibodies, three from IndianI (277, 903 and 904) and one from AmericanA (447-52D) HIV-1 infected donor were tested for their binding to overlapping peptides by ELISA using mAbs at a concentration ranging from 10 to 0.00003 μg/ml (12 dilutions).
  3. The 50% binding titers (Max50, conc. μg/ml) of each antibody against the corresponding peptides is depicted as numerical values in Bold (high affinity), Italic (low affinity) and >10, where Max50 value was not reached. Human anti-parvovirus B19 mAb 1418 was used as negative control. Each experiment was performed at least two independent times.
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Virology Journal

ISSN: 1743-422X