| |
1Amino acid sequence of C2-V3-C3 overlapping peptides
|
2anti-V3 mAbs
| |
|---|
|
Peptide ID
|
IIVHLNESVEIV CTRPNNNTRKSIRIGPGQTFYATGDIIGDIRQAHC NISEEKWNKTLQ
|
277
|
903
|
904
|
447-52D
|
1418
|
|---|
|
9256
|
IIVHLNESVEIVCTR
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9257
|
LNESVEIVCTRPNNN
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9258
|
VEIVCTRPNNNTRKS
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9259
|
CTRPNNNTRKSIRIG
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9260
|
NNNTRKSIRIGPGQT
|
>10
|
>10
|
>10
|
0.049
|
>10
|
|
9261
|
RKSIRIGPGQTFYAT
|
4.04
|
1.37
|
0.327
|
0.149
|
>10
|
|
9262
|
RIGPGQTFYATGDII
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9263
|
GQTFYATGDIIGDIR
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9264
|
YATGDIIGDIRQAHC
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9265
|
DIIGDIRQAHCNISE
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9266
|
DIRQAHCNISEEKWN
|
>10
|
>10
|
>10
|
>10
|
>10
|
|
9267
|
AHCNISEEKWNKTLQ
|
>10
|
>10
|
>10
|
>10
|
>10
|
- 1Amino acid sequences of linear overlapping peptides encompassing the third variable region (V3: Underlined (middle)), flanked by second (C2: (left)) and third (C3: (right)) constant regions and are aligned with the corresponding consensus-C gp120 sequence.
- 2Four anti-V3 antibodies, three from IndianI (277, 903 and 904) and one from AmericanA (447-52D) HIV-1 infected donor were tested for their binding to overlapping peptides by ELISA using mAbs at a concentration ranging from 10 to 0.00003 μg/ml (12 dilutions).
- The 50% binding titers (Max50, conc. μg/ml) of each antibody against the corresponding peptides is depicted as numerical values in Bold (high affinity), Italic (low affinity) and >10, where Max50 value was not reached. Human anti-parvovirus B19 mAb 1418 was used as negative control. Each experiment was performed at least two independent times.
