Table 1 Primers used for sequencing reactions
From: Isolation and characterization of a variant porcine epidemic diarrhea virus in China
Oligonucleotides | Locationa | sequence |
|---|---|---|
1 F | 190-209 | GCGTTCCGTCGCCTTCTACA |
1R | 2751-2729 | CAGGAATCTGGAAGACACTTGCA |
2 F | 2663-2684 | GTATTATGCCACCAGTGTCCCA |
2R | 4957-4938 | CAGTTGCCAGCAGGCACTGT |
3 F | 4887-4906 | ACCAGCGGTGCATTGCTTGA |
3R | 7475-7453 | CAATGTGCTCTTGCAATCCTGCA |
4 F | 7327-7350 | CTGTTAAGTTAGTGGACTCAGCGT |
4R | 9875-9856 | ACTAGCGCCTTCAACTTGCA |
5 F | 9712-9731 | GCGCTTGTGGTTCACCTGGT |
5R | 12259-12240 | GGATCCACAGCGAAAGCGCA |
6 F | 12182-12202 | ACGCTTGCAGGCTGGTAAACA |
6R | 14462-14442 | TGGGCAGTGCTCTATCGCACT |
7 F | 14322-14341 | ATACTAGGGGCGCTTCGGTT |
7R | 16780-16760 | GTCAGGGTGCACAGGAATGAA |
8 F | 16662-16684 | GTATGTGTGCCCTTAAGCCTGAT |
8R | 19002-18980 | GTAAGTGGACGTTCGGCTTCATA |
9 F | 18874-18898 | CGTAGCTTTTGAGTTGTATGCCA |
9R | 21330-21309 | GCAATTAGCTGTACAGGGTTCA |
10 F | 21080-21101 | CCATTCCAGCTTATATGCGTGA |
10R | 23487-23465 | GTACATGTGAAGCTTCTCAGCGT |
11 F | 23272-23292 | GTGTACGATCCTGCAAGTGGC |
11R | 25715-25694 | TCACCTCATCAACGGGAATAGA |
12 F | 25535-25557 | TCGTCCAATTGGTTAATCTGTGC |
12R | 27840-27820 | TACCGTTGTGTGCAAGACCAA |
5’ RACE Primer | 307-288 | TTGCTAGCCATAGCCGGCAG |
3’Race Primer | 27725-27747 | CTATGTTCCAGGGTAGTGCCATT |