Skip to main content

Table 2 Oligonucleotide primers used for site-directed mutagenesis and sequencing

From: Effects of amino acid substitutions in the VP2 B-C loop on antigenicity and pathogenicity of serotype Asia1 foot-and-mouth disease virus

Primer

Positionc

Sequenced

Orientation

154Fa

3704-3740

5'-CCCTCGCACGCAGAGTGAACAACCGGCTGCCCACTTC-3'

Sense

154Ra

3704-3740

5'-GAAGTGGGCAGCCGGTTGTTCACTCTGCGTGCGAGGG-3'

Antisense

72GFa

2148-2181

5'-GTTTGACTGGACGCCGGGTTTGTCATTTGGACAC-3'

Sense

72GRa

2148-2181

5'-GTGTCCAAATGACAAACCCGGCGTCCAGTCAAAC-3'

Antisense

72NFa

2148-2181

5'-GTTTGACTGGACGCCGAATTTGTCATTTGGACAC-3'

Sense

72NRa

2148-2181

5'-GTGTCCAAATGACAAATTCGGCGTCCAGTCAAAC-3'

Antisense

107Fa

2253-2287

5'-GAGGAACGGGTGGGACATTGAGGTGACCGCTGTTG-3'

Sense

107Ra

2253-2287

5'-CAACAGCGGTCACCTCAATGTCCCACCCGTTCCTC-3'

Antisense

S1Fb

207-227

5'-AACAGCTATGACCATGATTAC-3'

Sense

S1Rb

2677-2697

5'-GTTAGACACTTTCCCGTAGAC-3'

Antisense

S2Rb

4000-4019

5'-GACATGTCCTCCTGCATCTG-3'

Antisense

  1. a Primers for the construction of mutant viruses.
  2. b Primers for sequencing.
  3. c The position of primers was determined according to the sequence of pAsi.
  4. d The mutated codons are bolded and underlined.