Skip to main content

Advertisement

Table 2 Oligonucleotide primers used for site-directed mutagenesis and sequencing

From: Effects of amino acid substitutions in the VP2 B-C loop on antigenicity and pathogenicity of serotype Asia1 foot-and-mouth disease virus

Primer Positionc Sequenced Orientation
154Fa 3704-3740 5'-CCCTCGCACGCAGAGTGAACAACCGGCTGCCCACTTC-3' Sense
154Ra 3704-3740 5'-GAAGTGGGCAGCCGGTTGTTCACTCTGCGTGCGAGGG-3' Antisense
72GFa 2148-2181 5'-GTTTGACTGGACGCCGGGTTTGTCATTTGGACAC-3' Sense
72GRa 2148-2181 5'-GTGTCCAAATGACAAACCCGGCGTCCAGTCAAAC-3' Antisense
72NFa 2148-2181 5'-GTTTGACTGGACGCCGAATTTGTCATTTGGACAC-3' Sense
72NRa 2148-2181 5'-GTGTCCAAATGACAAATTCGGCGTCCAGTCAAAC-3' Antisense
107Fa 2253-2287 5'-GAGGAACGGGTGGGACATTGAGGTGACCGCTGTTG-3' Sense
107Ra 2253-2287 5'-CAACAGCGGTCACCTCAATGTCCCACCCGTTCCTC-3' Antisense
S1Fb 207-227 5'-AACAGCTATGACCATGATTAC-3' Sense
S1Rb 2677-2697 5'-GTTAGACACTTTCCCGTAGAC-3' Antisense
S2Rb 4000-4019 5'-GACATGTCCTCCTGCATCTG-3' Antisense
  1. a Primers for the construction of mutant viruses.
  2. b Primers for sequencing.
  3. c The position of primers was determined according to the sequence of pAsi.
  4. d The mutated codons are bolded and underlined.