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Figure 4 | Virology Journal

Figure 4

From: One-step detection of Bean pod mottle virusin soybean seeds by the reverse-transcription loop-mediated isothermal amplification

Figure 4

Sensitivity of the RT-LAMP vs conventional RT-PCR. (A) Serial 10-fold dilutions of total RNA of BPMV soybean seeds sample 1 extracted using Trizol Reagent were subjected to RT-LAMP from 100 to 0.0001 ng. 1–7: 100, 10, 1, 0.1, 0.01, 0.001, 0.0001 ng RNA was used as template, respectively; 8: No RNA template. (B) Total RNA same as (A) was used to one-step RT-PCR. (C) Crude RNA extract of sample 1 were used as templates in RT-LAMP from 0.2 to 2.0 μL at intervals of 0.2 μL. 1–10: 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0 μL RNA was used as template, respectively; 11: Healthy soybean seeds; 12: No RNA template. 3 μL of amplified products were checked on a 1% agarose gel electrophoresis. M: 2 kb DNA marker.

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