A sensitive and specific blocking ELISA for the detection of rabbit calicivirus RCV-A1 antibodies

Table 1 Comparison of RCV blocking ELISA (bELISA) and competition ELISA (cELISA)

Sample ID	Inhibition at dilution of 1:10 (%)^a	RCV bELISA titre	RCV cELISA^b titre	RHDV cELISA^b titre
RCV-1^c	57	160	640	0
RCV-2	56	160	160	0
RCV-3	65	160	40	0
RCV-4	60	320	160	80
RCV-8	42	40	80	0
RCV-9	62	160	320	0
RCV-10	56	160	0	0
RCV-11	72	160	40	0
RCV-12	63	160	20	0
RCV-13	50	160	0	0
RCV-14	51	160	0	0
RCV-15	53	160	0	0
RCV-16	71	160	640	0
RCV-17	59	80	640	0
RCV-18	70	160	80	0
RCV-19	61	160	80	0
RCV-20	43	40	0	0
RCV-21	55	40	0	0
RCV-22	58	40	640	0
RCV-23	66	160	160	0
RCV-24	74	320	160	0
RCV-25	66	160	320	0
RHDV-1	6	0	0	320
RHDV-2	0	0	0	81,960
RHDV-3	0	0	0	20,480
RHDV-4	10	0	0	640
RHDV-5	0	0	0	160
RHDV-6	0	0	0	160
RHDV-7	2	0	0	160
RHDV-8	2	0	0	320
RHDV-9	2	0	0	640
RHDV-W1^d	7	0	0	80
RHDV-W2	1	0	0	80
RHDV-W3	1	0	0	320
RHDV-W4	1	0	0	80
RHDV-W5	1	0	0	160
RHDV-W6	0	0	0	160
RHDV-W7	6	0	0	80
RHDV-W8	6	0	0	320
RHDV-W9	5	0	0	80
RHDV-W10	8	0	0	40
RHDV-W11	7	0	0	40
RHDV-W12	0	0	0	160
RHDV-W13	0	0	0	640
RHDV-W14	3	0	0	320
RHDV-W15	12	0	0	320
RHDV-W16	6	0	0	320
RHDV-W17	13	0	0	160
RHDV-W18	12	0	0	320
RHDV-W19	23	0	0	640
RHDV-W20	7	0	0	80
RHDV-W21	20	0	0	320
RHDV-W22	14	0	0	640
RHDV-W23	22	0	0	320
RHDV-W24	8	0	0	320
RHDV-W25	12	0	0	320

^aThe inhibition of sera tested at dilution of 1:10 in the blocking ELISA when RCV-VLP was used at 2.5 μg/ml (1:1500) and 0.33 μg/ml (1:4000), respectively. ^bRCV cELISA and RHDV cELISA were performed according to the RCV and RHDV cELISA-2 in Liu et al. [25]. ^cData for RCV cELISA and RHDV cELISA of sera RCV-1 to RHDV-9 are from Liu et al. [25] for comparison. ^dSera RHDV-W1 to W25 were sampled from wild rabbit populations proven to be free of RCV-A1 by quantitative PCR and serological tests.