Figure 4From: Two types of nanoparticle-based bio-barcode amplification assays to detect HIV-1 p24 antigenAmplification detection of 10-fold diluted bio-barcode is revealed by 4% agarose gel electrophoresis. The concentration of DNA in lane 1 was 0.005 uM and 5 μl of PCR DNA were used for gel electrophoresis. Lane M is the 20 bp ladder reference DNA marker (Takara Inc.). Lanes 1 to 9 represents amplicons 3 × 109, 3 × 108, 3 × 107, 3 × 106, 3 × 105, 3 × 104, 3 × 103, 300, and 30 copies of signal bio-barcode DNA. Lane 10 represents negative control (PCR reaction mixture without input DNA).Back to article page