Figure 2From: NDV-induced apoptosis in absence of Bax; evidence of involvement of apoptotic proteins upstream of mitochondriaNDV infection leads to translocation of Bax and release of cytochrome c into cytosol in colon cancer cells. Infected wt HCT116, HCT116 Bax −/− and HT29 cells (18 h, 36 h and 48 h post-infection) were subjected to subcellular fractionation. The cytosolic samples were analyzed by Western blotting with anti-Bax clone 2D2 and anti-cytochrome c clone 7H8 antibodies. Absence of Bax in HCT116 Bax−/− cells was also checked.Back to article page