Figure 6
From: Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis
NS1 upregulates expression of the rescued S10-Rluc reporter segment in replicating BTV-1 S10Rluc. (A) 96 well monolayers were transfected with 100 ng pCAG NS1 or pCAGGS vector, and infected with BTV-1 S10Rluc. The amount of luciferase was assayed in triplicate at 1 h intervals following infection with the BTV-1 S10Rluc reporter virus. Values are relative light units (RLU) expressed as the mean of three replicates. (B) Graph of the fold increase of the virus expressed reporter in NS1 expressing cells compared to vector transfected BSR. Values are the fold increase observed when NS1 was pre-expressed. Bars indicate one standard deviation from the mean.