Figure 4
From: Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis
Expression from capped S10-Rluc RNA is upregulated by NS1. (A) pS10-Rluc contains the 5′ 152nt and 3′ 149nt of BTV-10 segment 10 fused with the Renilla luciferase gene. (B) BSR 96 well monolayers transfected with 100 ng pCAG NS1 and capped S10-Rluc RNA mutants. The amount of luciferase present in the transfected cell lysates was assayed in triplicate at 20 h after transfection with S10-Rluc reporter RNA. Bars indicate one standard deviation from the mean.