Figure 3
From: Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis
NS1 does not act through NS3 sequences in the reporter protein. (A) pS10-GFP mutants contain point mutations which reduce or eliminate the regions of NS3 fused to GFP. Mut1, introduced termination codon and −1 frame shift at C-terminus of GFP. Mut2, NS3 initiation codon destroyed. Mut 4, NS3 initiation codon and 2nd methionine codon destroyed. Mut5, NS3 initiation codon and 2nd methionine codon destroyed, and at the C-terminus of GFP a termination codon and −1 frame shift were introduced. (B) BSR 96 well monolayers transfected with 100 ng pCAG NS1 and capped S10-GFP RNA mutants. Fluorescence was recorded at 20 h after transfection with reporter RNAs.