Figure 1
From: Bluetongue virus non-structural protein 1 is a positive regulator of viral protein synthesis
Expression of BTV genes upregulates expression from capped S10-GFP RNA. (A) pS10-GFP contains the 5′ 152nt and 3′ 149nt of BTV-10 segment 10 fused in frame with GFP. The 5′ end of the capped S10-GFP reporter RNA is defined by the T7 promoter (italicised) and the 3′ end is determined by a Bsm BI site (underlined). (B) and (C) Confluent 24 well BSR monolayers fixed in 4% PFA at 22 h post-transfection. (B) Transfected with 1 μg capped S10-GFP reporter RNA alone. (C) Transfected with 1 μg capped S10-GFP reporter RNA + 0.8 μg BTV-1 mRNA produced in vitro from transcribing cores as previously described[35].