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Figure 4 | Virology Journal

Figure 4

From: In vitroinfection with classical swine fever virus inhibits the transcription of immune response genes

Figure 4

Comparison of deferentially expressed CD40 using real-time PCR and Western blot. (a) mRNA changes of CD40 gene in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test). (b) Detection of changes in protein expression by western blot with specific antibodies against CD40, β-tubulin, and CSFV glycoprotein E2 in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. The dimer of E1 and E2 (E1/E2) was detected with specific antibody against CSFV glycoprotein E2. (c) The quantification of changes in protein expression by the former western blot. The relative expression levels of CD40 were compared with that of β-tubulin. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test).

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Virology Journal

ISSN: 1743-422X