Figure 3

Comparison of deferentially expressed SLA-DR by real-time PCR and Western blot analysis. (a) mRNA changes of SLA-DR gene in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test). (b) Detection of changes in protein expression by western blot with specific antibodies against SLA-DR, β-tubulin, and CSFV glycoprotein E2 in PK-15 cells following CSFV infection at 1, 3, 12, 24, 36, and 48 hpi. The dimer of E1 and E2 (E1/E2) was detected with specific antibody against CSFV glycoprotein E2. (c) The quantification of changes in protein expression by the former western blot. The relative expression levels of SLA-DR were compared with that of β-tubulin. (Note: Bars above columns represent standard deviations. * represents the significant difference (p<0.001) in comparison with the control by t-test).