Figure 3

The MAb against PHE-CoV were identified by Western blot analysis. Lane 1: Prestained Protein Marke (TaKaRA, code: 530A, Dalian, P. R. China,) Lane 2: The purities of MAb 1E2 were used in Western blot analysis, which recognizes the S Protein of PHE-CoV. Lane 3: The purities of MAb 4D4 were used in Western blot analysis, which recognizes the HE Protein of PHE-CoV. Lane 4: The SP2/0 cells was injected into Balb/C nude mice and the mouse abdominal dropsy, and the purities were used negative control.