Genomic sequencing and analyses of HearMNPV—a new Multinucleocapsid nucleopolyhedrovirus isolated from Helicoverpa armigera

Tang, Ping; Zhang, Huan; Li, Yinü; Han, Bin; Wang, Guozeng; Qin, Qilian; Zhang, Zhifang

doi:10.1186/1743-422X-9-168

Virology Journal

Table 1 List of ORFs in HearMNPV and their Homologous ORFs in the MacoNPV-B, MacoNPV-A, HearSNPV(G4), AcMNPV, AgSeNPV and HearGV

From: Genomic sequencing and analyses of HearMNPV—a new Multinucleocapsid nucleopolyhedrovirus isolated from Helicoverpa armigera

ORF	Name	Position^a			Prm^b	length	Homologous ORFs [ORF number,amino acid identity(%)]
ORF	Name	Position^a			Prm^b	length	MacoNPV-B		MacoNPV-A		HearSNPV(G4)		AcMNPV		AgSeNPV		HearGV
1	polh	1	>	741	L	246	1	100	1	98.0	1	87.4	8	89.3	1	89.8	1	55.1
2	1629	790	<	2358	L	522	2	95.8	2	72.3	2	26.6	9	27.9	2	33.0	2	16.7
3	pk1	2357	>	3175		272	3	99.6	3	87.5	3	48.5	10	38.7	3	58.6	3	34.6
4	hoar	3250	<	5478	E	742	4	97.1	4	74.3	4	20.2			4	27.7
5		5967	>	6533	E	188	5	96.8	5	67.2
6	odv-e56	6626	>	7747	L	373	6	100	6	89.3	15	52.6	148	49.2	6	59.5	14	42.1
7	me53	7892	<	8956		354	7	99.7	7	86.2	16	30.9	139	24.6	7	47.4	178	22.1
8	F protein	9563	>	11599	L	678	8	99.9	9	90.9	133	36.9	23	22.1	8	44.4	26	29.8
9		11719	<	12678	E	319	9	99.1	10	91.2					9	46.1
10	gp16	12729	<	13016	L	95	10	100	11	97.9	119	30.8	130	35.5	10	65.6
11	p24	13029	<	13718	L	229	11	100	12	92.5	118	50.4	129	37.4	11	63.8	78	25.7
12		13784	>	14095	L	103	12	100	13	91.3	117a	34.6			12	44.4
13	lef2	14049	>	14696		215	13	99.1	14	82.0	117	47.9	6	40.6	13	56.6	33	26.8
14	xe	14792	>	15175	E	127	14	99.2	15	85.5							21	22.0
	hr1	15251		16435
15	endonuclease	16436	<	16732	L	98	15	99.0	17	93.4			79	44.7			69	40.5
16		16798	>	17397		199	16	98.5	18	87.9			70	23.9	25	27.0
17		17550	>	18260	E,L	236	117	41.2	19	80.6			151	33.0	103	38.9
18	chitinase	18318	<	20006	L	562	19	99.3	22	97.0	41	63.3	126	67.2	23	82.6	105	60.3
19	bro-a	20197	>	21192		331	20	83	24	77.7	60	53.9	2	23.3	50	33.2	101	64.3
20		21257	>	21682	E	141	21	99.3	25	86.5							131	38.9
21		21786	>	22595	E	269	22	98.1	26	78.1							52	40.8
22		22706	<	23347		213	23	95.8	27	91.1	57	25.8			49	21.4	81	34.9
23		23525	>	23839	L	104	24	99.0	29	64.4
24		23960	<	24595	E	211	25	97.6	30	69.2
25	hel2	25012	<	26379	E	455	26	98.9									147	57.7
26	he65	26512	<	28266		584	27	98.5	32	91.7	61	28.6	105	37.1	20	53.3	62	53.4
27	cathepsin	28331	>	29356		341	28	100	33	97.1	56	46.7	127	56.9	19	81.3
28		29353	<	29700	L	115	29	100	34	90.6	125	29.0			18	72.0
29	lef1	29728	>	30375		215	30	99.1	35	95.8	124	48.2	14	40.0	17	64.2	80	36.6
30	38.7 k	30375	>	31424	L	349	31	99.7	36	92.6	123	34.1	13	29.5	16	60.3	102	23.4
31	gp37	31476	>	32264	L	262	32	99.6	37	95.0	58	62.8	64	58.6	26	72.4	109	43.1
32	ptp2	32221	<	32760	L	179	33	98.9	38	92.7					27	51.0
33	egt	32828	>	34414	E	528	34	99.4	39	94.4	126	50.7	15	48.0	28	73.1
34		34583	>	35119	E	178	35	98.9	40	94.9	127	22.3			29	53.3
35		35119	>	35766		215	36	98.1	41	90.7	128	28.1	17	30.4	30	45.9
36		35802	<	38357		851	37	99.4	42	88.5	129	26.5			31	45.4
37	chtB2	38415	>	38855	L	146	38	98.6	43	64.6	83	31.7	146	43.8	148	34.0	107	46.2
38		38886	>	39413	L	175	39	99.4	44	82.8			4	26.7	32	36.8
39	pkip	39434	>	39943	L	169	40	100	45	91.6	130	36.2	24	23.5	33	55.6
40		39965	<	40306		113	41	100	46	96.5					34	52.7
41	arif1	40312	<	41184		290	42	98.6	47	92.0	131	29.2	21	22.7	35	43.3
42	pif2	40940	>	42199		419	43	99.3	48	95.0	132	69.0	22	59.5	36	74.6	42	51.1
43	pif1	42214	>	43803		529	44	99.6	49	93.6	111	43.0	119	48.6	37	61.2	82	36.2
44		43800	>	44045		81	45	100	50	96.3	112	32.9			38	44.3
45	fgf	44080	<	45174		364	46	99.2	51	72.9	113	32.0	32	27.5	39	41.7	176	34.0
46		45210	>	46115		301	47	99.7	53	89.5					40	45.7
47	alk-exo	46157	<	47344	L	395	48	99.8	54	90.1	114	41.4	133	38.8	41	48.6	146	39.4
48		47582	<	47920	L	112	49	100	55	93.6	115	27.0	19	26.7	44	65.1
49		47919	>	49082	L	387	50	99.7	56	94.6			18	25.8	45	62.3
50		49121	<	49522		133	51	99.3	57	94.7	122	22.5	132	21.9	46	50.0
51	rr2	49594	>	50535		313	52	99.7	58	94.3					47	72.2
52		50544	<	51593		349	53	100	59	73.1					103	55.3
53	calyx/pep	51620	<	52597		325	61	100	60	97.5	120	41.6	131	32.3	49	65.7	18	21.0
54		52871	<	53209		112	62	100	62	84.1	110	40.2	117	34.2	51	66.0
55		53161	<	53523	E	120	63	100	63	90.8	109	35.8			52	53.2
56		53687	<	54301	E,L	204	64	98.5	65	86.8
57	sod	54366	<	54821		151	65	100	66	96.7	106	75.3	31	73.2	54	85.2	63	53.7
58		54878	>	55243		121	66	100	67	86.8					55	47.3
59	pif3	55269	>	55880	L	203	67	98.0	68	92.6	98	51.8	115	47.1	56	70.0	30	44.5
60		55846	>	56316		156	68	98.1	69	87.7	99	22.7			57	57.5
61	pagr	56365	>	57819	L	484	69	99.4	70	87.4	100	23.5			58	38.0
62		57842	>	58483	L	213	70	100	71	96.8	101	57.1	106	53.2	59	78.2	45	44.1
63	nrk1	58518	<	59627	E	369	71	99.4	72	95.0			33	30.1	60	58.4
	hr2	59758		61523
64		61569	>	62045	L	158	72	93.7	73	87.3			4	25.6	32	20.2
65	dutpase	62105	>	62449	E	114	73	91.2	74	92.2					62	66.7
66		62531	>	64309	E,L	592	18	99									53	51.0
67	bro-b	64565	>	65572		335	74	77.5	75	79.6	60	47.5	2	23.0	50	32.0	101	54.8
68	p13	65621	>	66457	L	278	75	99.6	76	95.3	97	58.4			64	68.5	40	47.4
69	sprT	66512	>	67036		174	76	98.9	77	89.7							21	30.7
70	odv-e66a	67148	>	69166	E,L	672	77	99.7	78	97.2	96	58.1	46	39.7	125	35.9	150	56.3
71		69163	<	69474	L	103	78	99.0	79	96.1	95	44.7	108	32.8	65	59.6
72	odv-ec43	69484	<	70554	L	356	79	100	80	95.2	94	50.4	109	41.6	66	77.3	48	31.9
73		70538	<	70717		59	80	100	81	98.3	93	50.0	110	36.0	67	73.7	46	50.0
74	vp80	70714	<	72360		548	81	99.3	82	83.1	92	24.0	104	22.9	68	37.2
75	p45	72388	>	73521	E,L	377	82	99.7	83	97.9	91	58.7	103	51.1	69	80.7	90	39.5
76	p12	73508	>	73816	L	102	83	100	84	89.2	90	42.1	102	32.6	70	51.9
77	p40	73842	>	74936	L	364	84	100	85	92.6	89	53.1	101	43.4	71	69.6	92	21.6
78	p6.9	74995	>	75228	L	77	85	100	86	83.3	88	58.9	100	62.0	72	64.2	93	16.7
79	lef5	75225	<	76046		273	86	99.6	87	96.3	87	50.3	99	57.4	73	77.6	94	43.0
80	38 k	75945	>	76847	L	300	87	99.7	88	96.0	86	53.7	98	44.6	74	73.0	95	40.5
81	vef	76886	>	79432	L	848	88	99.3	89	81.3					76	39.5	151	28.4
82	bro-c	79437	<	80507		356	89	98.3	90	84.4	105	28.9	2	50.1	77	63.7	117	25.1
83		80591	<	81019		142	90	99.3	91	95.8					78	72.5
84	odv-e28	81052	<	81570		172	91	98.84	92	97.7	85	62.9	96	50.6	79	80.2	96	40.5
85	helicase	81527	>	85156	L	1209	92	99.83	93	96.5	84	46.8	95	41.8	80	78.7	97	27.8
86	odv-e25	85256	<	85906	L	216	93	100	94	94.9	82	64.5	94	45.1	81	83.9	98	48.0
87		85903	<	86388	L	161	94	100	95	98.8	81	69.7	93	51.0	82	88.4	99	32.9
88		86387	>	87145		252	95	99.6	96	94.8	80	57.5	92	51.4	83	82.5	100	37.9
89		87255	>	87770	E,L	171	96	97.7	97	91.2	77	33.9	142	34.7	84	31.7
90	lef4	87802	<	89166		454	97	99.6	98	91.9	79	48.4	90	44.5	85	66.3	112	35.3
91	vp39	89165	>	90154	L	329	98	98.5	99	79.6	78	50.9	89	42.1	86	55.2	113	31.9
92	cg30	90237	>	91061	E	274	99	97.8	100	80.8	77	21.2	88	21.7	87	28.6
93	91 k	91117	<	93555	L	812	100	99.3	101	92.9	76	44.4	83	41.5	88	58.4	121	28.3
94	tlp-20	93524	>	94111	L	195	101	98.0	102	91.9	75	41.4	82	31.4	89	63.7	122	41.2
95		93936	>	94658	L	240	102	99.2	103	82.8	74	62.3	81	49.5	90	68.3	123	51.5
96	gp41	94627	>	95628	L	333	103	100	104	97.9	73	55.7	80	57.1	91	80.4	124	36.0
97		95508	>	95963		151	104	100	105	92.2	72	41.5	78	35.9	92	58.3
98	vlf-1	95965	>	97107	L	380	105	100	106	97.4	71	62.8	77	64.0	93	90.5	126	27.6
99	ctl	97104	<	97256	L	50	106	98	107	94.0			3	49.0			130	65.3
100		97329	<	98423	E	364	107	98.1	108	82.7	34	22.7			127	22.5
101	p26	98524	<	99258	E	244	108	100	109	97.1	22	27.9	136	31.6	94	66.0
102	iap-2	99307	<	100053		248	109	99.2	110	87.8	62	43.1	71	32.8	95	57.6	139	27.6
103		100010	<	100825		271	110	98.9	111	92.6	63	48.5	69	45.4	96	67.1
104		100809	<	101174		121	111	100	112	95.9	64	55.1	68	47.9	97	75.6	137	40.4
105	lef3	101173	>	102354		393	112	99.5	113	82.2	65	30.5	67	27.0	98	54.3
106	desmoplakin	102414	<	104675		753	113	99.2	114	79.9	66	24.9	66	22.3	99	29.9	135	38.2
107	DNA pol	104674	>	107676		1000	114	99.9	115	94.3	67	59.3	65	45.5	100	72.5	134	38.3
108		107710	<	108099	L	129	115	100	116	99.2	69	40.5	75	26.4	101	84.5
109		108110	<	108367	L	85	116	100	117	100.0	70	70.6	76	41.9	102	87.1	128	32.9
110		108459	>	109199		246	117	97.2	118	86.0			151	61.5	103	39.0	10	32.9
111		109191	<	109736		181	118	98.9	119	87.9	57	31.8			49	23.6	81	29.5
112		109771	>	110232		153	119	98.7	120	96.1
113		110287	>	110934	L	215	120	96.7	121	88.8					104	46.6	167	30.4
114	bro-d	110975	<	112033		352	121	89.6	122	83.6	105	23.4	2	45.1	123	39.3	117	27.9
115	bro-e	112087	<	112776		229	122	97.4	123	76.5	59	25.0	2	34.4	77	28.4	133	34.3
116	lef9	112859	<	114352	L	497	123	99.6	124	96.4	55	70.2	62	64.5	105	85.9	140	53.5
117	fp25	114430	>	115017	L	195	124	100	125	99.0	53	71.2	61	62.8	106	88.7	141	33.6
118	p94	115094	>	117598		834	125	99.4	126	82.5			134	41.7			20	35.8
119	bro-f	117622	>	118161	L	179	126	98.8	127	95.0	60	42.3			107	64.0	158	50.9
120	chaB2	118194	>	118469	L	91	127	100	128	96.7	52	55.2	60	48.8	108	67.4	103	38.6
121	chaB1	118447	>	118956		169	128	94.4	129	87.4	51	39.0	59	49.1	109	53.8
122		118949	<	119428	E	159	129	98.7	130	95.0	50	40.3	57	37.4	110	59.4
123		119678	<	119947	L	89	130	100	131	92.1	49	55.6	56	42.9	111	60.0
124		119889	<	120098		69	131	100	132	95.8	48	49.3	55	42.0	112	71.6
125	vp1054	120224	<	121234	E,L	336	132	100	133	93.2	47	53.2	54	40.3	113	67.3	173	35.0
126	lef10	121095	<	121322	L	75	133	98.7	134	93.3	46	47.2	53a	50.7	114	62.7
127		121282	>	121509	L	75	134	100	135	93.3	45	36.0			115	50.8
128		121523	>	122509	L	328	135	97.3	136	75.0	44	29.0			116	34.0
129		122514	<	122987	L	157	136	100	137	94.3	43	57.4	53	51.2	117	66.9	169	28.8
130		122986	>	123489		167	137	100	138	88.6	42	26.5	52	20.8	118	49.1
	hr3	123528		124601
131	Iap3	124860	>	125717	L	285	138	99.3	139	82.5	103	33.2	27	28.0	119	43.9	139	25.5
132	bjdp	125756	<	126910		384	139	98.7	140	88.0	39	31.5	51	19.5	120	36.6
133	lef8	126931	>	129567		878	140	99.9	141	97.3	38	68.0	50	60.5	121	78.9	149	50.3
134		129595	<	130059		154	141	98.7	142	82.2					122	29.7
135		130103	<	130303		66	142	100	143	95.4	37	25.4	43	32.3	124	58.6
136	odv-e66b	130351	<	132342	L	663	143	98.6	144	88.8	96	33.8	46	27.9	125	50.2	150	35.4
137	p47	132390	>	133583		397	144	99.8	145	97.2	35	61.8	40	55.2	126	75.6	74	46.6
138		133594	<	134643		349	145	99.1	146	85.6					127	34.0
	hr4	134722		135445
139		135447	<	135710	E	87
140		135947	>	136519	E	190	146	98.4	147	81.9					129	30.8
141	bv-e31	136581	>	137285	E,L	234	147	100	148	94.9	33	66.5	38	63.3	130	85.0	77	42.4
142	lef11	137210	>	137584	L	124	148	99.2	149	90.2	32	49.0	37	39.1	131	64.6	51	38.1
143	39 k	137553	>	138407	L	284	149	99.3	150	92.0	31	30.3	36	33.8	132	49.8	50	30.2
144		138476	<	138673		65	150	98.5	151	86.2					134	37.1
145	ubiquitin	138600	<	138902	L	100	151	100	152	93.9	28	77.6	35	77.9	135	94.7	47	76.6
146		138958	>	139503	L	181	152	98.9	153	93.4	27	50.3	34	35.5	136	63.8
147		139854	<	140210	L	118	153	99.2	154	94.9	26	36.6	26	32.4	138	61.1
148	dbp-2	140299	>	141279	E	326	154	99.1	155	95.4	25	42.8	25	24.2	139	59.9	87	24.0
149	lef6	141285	>	141710	L	141	155	98.6	156	93.7	24	45.7	28	25.5	140	47.1
150		141751	<	141996		81	156	100	157	97.5			29	41.4	141	75.3
151	p26	142112	>	142912	L	266	157	98.9	158	96.6	22	44.9	136	33.1	142	67.1
152	p10	142951	>	143202	L	83	158	100	159	92.9	21	47.1	137	35.1	143	70.4	5	43.4
153	p74	143289	<	145262	L	657	159	99.4	160	94.7	20	55.3	138	52.5	144	65.9	72	39.3
154		145343	>	145594	E,L	83	160	98.8	161	91.8	19	32.1			145	53.0
155	ie1	145631	<	147436		601	161	99.3	162	91.1	14	41.5	147	29.4	146	47.4
156	ep23	147478	>	148053	L	191	162	100	163	95.3	13	31.4	146	33.5	147	63.9	9	30.9
157	chtB1	148114	<	148392	L	92	163	100	164	92.4	12	55.4	145	44.2	148	70.7	10	40.7
158	odv-e27	148395	<	149231	L	278	164	100	165	97.5	11	59.8	144	51.7	149	90.7	114	27.7
159	odv-e18	149270	<	149527	L	85	165	100	166	88.4	10	54.1	143	56.3	150	62.1	11	61.1
160	p49	149529	<	150914	L	461	166	100	167	97.4	9	57.4	142	49.2	151	78.0	12	35.9
161	ie0	150932	<	151636	L	234	167	99.6	168	95.7	8	42.7	141	30.8	152	64.5
162	rr1	151802	<	154087	E	761	168	99.5	169	93.3					153	62.3

Note.^aColumn 3 indicates ORF location and transcriptional direction on the HearMNPV genome. ^bColumn 4 indicates the presence of early (E) and/or late (L) promoters located upstream of the start codon of each ORF. E indicates a TATA sequence followed by a CAGT or CATT mRNA start site sequence 20–40 nucleotides downstream, within 180 bp upstream of the start codon. L indicates the presence of a (A/T/G) TAAG up to 180 bp upstream of the initiation codon.

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