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Figure 6 | Virology Journal

Figure 6

From: Influenza A H3N2 subtype virus NS1 protein targets into the nucleus and binds primarily via its C-terminal NLS2/NoLS to nucleolin and fibrillarin

Figure 6

Chimeric GFP-NS1 A/Udorn/72/(203–237) colocalizes with HIV-1 Rev protein in the nucleolus, and a high expression level of HIV-1 Rev protein totally displaced the GFP-NS1(203–237) fusion protein from the nucleoli to the nucleus. HuH7 cells were grown on coverslips for 24 h and then transiently transfected with HIV-1 Rev and chimeric GFP-NS1 A/Udorn/72/(203–237) gene constructs for 48 h. After fixation, the cells were stained with rabbit anti-HIV-1 Rev and Rhodamine Red-X -labeled goat anti-rabbit immunoglobulins, followed by analysis with confocal laser microscopy. A high expression level of HIV-1 Rev protein totally displaced the GFP-NS1(203–237) fusion protein from the nucleoli to the nucleus (bottom panels). Cells, expressing low, medium or high amounts of nucleolar HIV-1 Rev protein, were selected. Only the nuclei of the cells are presented. Bar, 5 μm.

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Virology Journal

ISSN: 1743-422X