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Figure 3 | Virology Journal

Figure 3

From: Induction of interleukin-10 is dependent on p38 mitogen-activated protein kinase pathway in macrophages infected with porcine reproductive and respiratory syndrome virus

Figure 3

PRRSV-stimulated IL-10 production was selectively inhibited by inhibitors of p38 MAPK and NF-κB signaling pathways. BMDMs were pretreated with DMSO or PI3K inhibitor LY294002, ERK MAPK inhibitor PD98059, p38 inhibitor SB203580, and NF-κB inhibitor BAY11-7082 at the indicated concentrations for 2 h. Cells were then infected with PRRSV (MOI = 1) and harvested at 24 h.p.i.. (a) Total RNA isolated from cells was reverse transcribed and then analyzed using real-time PCR. (b) IL-10 production in cell supernatants was analyzed by ELISA. Data represent means ± SD of three independent experiments. *Significant difference (P < 0.05) from PRRSV control using Student’s t-test.

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