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Table 4 Type-specific primers for the detection of HPV types 30, 74, 86 and 90

From: Next-generation sequencing of cervical DNA detects human papillomavirus types not detected by commercial kits

HPV type

Primer name

Sequence

Target

Amplicon size (bp)

30

HPV30F

TGAGGTACAAGAAACATCGTTGC

158-180a

346

HPV30R

CGTACGTGATATTCTGTGAAACC

503-481a

74

HPV74F1

TGCTGGACAACATGCATGGAAAAATCCTAC

418-448 d

328

HPV74R1

CCTCTGTACCTGTATTTTCCGCCATGT

745-719 d

86

HPV86F

GTTTGTAGGAGTGTGGCATCC

29-10b

236

HPV86R

TTGTACTGCCAAGTTTCTGTCC

7781-7802b

90

HPV90E6F

GCGGAACAGCATTAACAGAGGA

95-116 c

258

HPV90E6R

TAAGCCGTTCCTTTTCCTCACT

352-331 c

  1. aPosition of primers within the E6 gene of HPV 30 (X74474), bHPV 86 (AF349909) and cHPV 90 (AY057438).
  2. dPosition of primers in the E6 and E7 genes of HPV 74 AE10 subtype (AF436130).