Figure 6

HBx promotes proliferation ability of HepG2 and Huh-7 cells through upregulation of LASP-1. (A). After transfection with siRNA targeting LASP-1 and incubation for 24 h, the cell proliferation was examined by Cell viability assay. Data were presented as mean ± SD of the absorbance value of cells. (B) HepG2-HBx and Huh-7-HBX cells were treated with siRNA targeting LASP-1 for 24 h. Colony formation efficiency was detected by plate clone formation assay. After incubation for two weeks, the cells were stained with Crystal Violet Staining Solution and observed using Bio-Rad Gel Doc XR documentation system. HepG2-Mock and Huh-7-Mock groups served as control groups. HepG2-HBX and Huh-7-HBX groups were HBX stable expressing cells. siRNA-control group was HepG2-HBX or Huh-7-HBX cell transfected with pcDNA™6.2-GW/EmGFP-miR vector as siRNA negative control cell. siRNA-LASP-1 group was HepG2-HBX or Huh-7-HBX cells transfected with siRNA targeting LASP-1 plasmids. *P < 0.05 compared with control cells. ^#P < 0.05 compared with HBx stable expressing cells. *P < 0.05 compared with HBx stable expressing cells transfected with siRNA negative control plasmids.