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Figure 4 | Virology Journal

Figure 4

From: Low-level HIV infection of hepatocytes

Figure 4

HIV infection of primary hepatocytes. (A) Primary human hepatocytes were incubated with DNase-treated HIVNL4-3 for two hours. After 24 hours, integrated HIV DNA was quantified in cell lysates by nested real-time PCR. (B)TZM-bl indicator cells were incubated with culture supernatants collected at day 3 post-infection from primary human hepatocytes infected with HIVNL4-3 or 100 ng AT-2 treated HIVNL4-3 and tested for β-galactosidase activity. The mean number of positive TZM-bl cells per well is shown with the error bars representing the standard deviation between duplicates. β-gal expression was also evaluated in the presence of 100 uM AZT for one hour prior to and during infection.

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