Figure 3
Infectious HIV production in hepatocytes. TZM-bl indicator cells were incubated with culture supernatants collected at day 3 post-infection from Huh7.5 or Huh7.5JHF1 cells (A) or Jurkat cells (B) infected with HIVNL4-3 or 100 ng AT-treated HIVNL4-3and tested for β-galactosidase activity. The mean number of positive TZM-bl cells per well is shown with the error bars representing the standard deviation between duplicates. β-gal expression was also evaluated in the presence of 100 uM AZT for one hour prior to and during infection.