Figure 1
Detection of integrated HIV DNA in hepatocytes. Huh7.5 (A), Huh7.5JFH1(B), or Jurkat (D) cells were incubated in the presence of 0, 1, 10, 100, or 1000 uM of the integrase inhibitor raltegravir for one hour prior to and during infection with DNase-treated HIVNL4-3. Huh7.5 (C) cells were incubated in the presence of raltegravir for one hour prior to and during infection with DNase-treated HIVYK-JRCSF. After 24 hours, integrated HIV DNA was quantified in cell lysates by nested real-time PCR. Error bars represent the standard deviation between duplicates.