Protein production and viral protection study. (A) Sedimentation profiles of purified NPfl and NPt-VP11-100 proteins were analyzed on a 12 % SDS-PAGE gel stained with Coomassie Brilliant Blue. * indicates the fractions that were pooled and concentrated. (B) Hamster's physiology before and after viral challenge. Arrow indicates limb paralysis. (C) Paralysis score and (D) body weight of hamsters following challenge with EV71P5.